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Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit | IGFBP6 elisa kit

Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit

Gene Names
IGFBP6; IBP6
Reactivity
Sheep
Synonyms
Insulin Like Growth Factor Binding Protein 6; Sheep Insulin Like Growth Factor Binding Protein 6 ELISA Kit; IGFBP6 elisa kit
Ordering
For Research Use Only!
Reactivity
Sheep
Specificity
No significant cross-reactivity or interference between Sheep IGFBP6 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
6.25ng/ml-200ng/ml.
Sensitivity
1.0ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IGFBP6 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep IGFBP6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGFBP6 and IGFBP6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGFBP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for IGFBP6 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IGFBP6 concentrations in Sheep serum, plasma and other body fluids. Using Purified Sheep IGFBP6 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IGFBP6 and IGFBP6 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IGFBP6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
25,322 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 6
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 6
NCBI Official Symbol
IGFBP6
NCBI Official Synonym Symbols
IBP6
NCBI Protein Information
insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6
UniProt Protein Name
Insulin-like growth factor-binding protein 6
UniProt Gene Name
IGFBP6
UniProt Synonym Gene Names
IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
UniProt Entry Name
IBP6_HUMAN

Uniprot Description

IGFBP6: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi apparatus; extracellular space; extracellular region

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: negative regulation of cell proliferation; cellular protein metabolic process; regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth; signal transduction

Research Articles on IGFBP6

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Product Notes

The Sheep IGFBP6 igfbp6 (Catalog #AAA024974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA024974 ELISA Kit recognizes Sheep IGFBP6. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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