Goat Insulin Like Growth Factor Binding Protein 6 ELISA Kit | IGFBP6 elisa kit

Goat Insulin Like Growth Factor Binding Protein 6 ELISA Kit

Cat. Num. AAA741994

• Gene Names: IGFBP6; IBP6
• Reactivity: Goat
• Synonyms: Insulin Like Growth Factor Binding Protein 6; Goat Insulin Like Growth Factor Binding Protein 6 ELISA Kit; IGFBP6 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP6 elisa kit

Intended Uses: This cIGFBP6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat IGFBP6. This ELISA kit for research use only!

Principle of the Assay: IGFBP6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGFBP6 antibody and an IGFBP6-HRP conjugate. The assay sample and buffer are incubated together with IGFBP6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGFBP6 concentration since IGFBP6 from samples and IGFBP6-HRP conjugate compete for the anti-IGFBP6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGFBP6 from the sample, fewer sites are left to bind IGFBP6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGFBP6 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IGFBP6 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 11321593
• NCBI GeneID: 3489
• NCBI Accession #: NP_002169.1
• NCBI GenBank Nucleotide #: NM_002178.2
• Molecular Weight: 25,322 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 6
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 6
• NCBI Official Symbol: IGFBP6
• NCBI Official Synonym Symbols: IBP6
• NCBI Protein Information: insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6
• UniProt Protein Name: Insulin-like growth factor-binding protein 6
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP6
• UniProt Synonym Gene Names: IBP6; IBP-6; IGF-binding protein 6; IGFBP-6
• UniProt Entry Name: IBP6_HUMAN

Uniprot Description

IGFBP6: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: Golgi apparatus; extracellular space; extracellular region

Molecular Function: insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: negative regulation of cell proliferation; cellular protein metabolic process; regulation of insulin-like growth factor receptor signaling pathway; regulation of cell growth; signal transduction