Rabbit anti-Human NCOA3 / SRC-3 / AIB1 Polyclonal Antibody | anti-NCOA3 antibody

Rabbit Polyclonal to Human NCOA3 / SRC-3 / AIB1

Cat. Num. AAA248140

• Gene Names: NCOA3; ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NCOA3 / SRC-3 / AIB1; Polyclonal Antibody; Rabbit Polyclonal to Human NCOA3 / SRC-3 / AIB1; Anti-NCOA3 / SRC-3 / AIB1 Antibody (Internal) IHC-plus; NCOA3; ACTR; AIB1; BHLHe42; CAGH16; CBP-interacting protein; CTG26; AIB-1; P/CIP; TNRC14; TRAM-1; Nuclear receptor coactivator 3; PCIP; RAC-3; SRC3; TNRC16; KAT13B; NCoA-3; SRC-3; Human NCOA3; anti-NCOA3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This antibody reacts with several isoforms of human NCOA3 protein (a, b, d, e and f). A BLAST analysis was used to suggest cross-reactivity with NCOA3 from human and macaque sources based on a 100% homology with the immunizing sequence. Expect partial reactivity with NCOA3 from dog, bovine, mouse and rat sources based on partial sequence homology. Cross-reactivity with NCOA3 from other sources has not been determined.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Sequence Length: 1420

• Applicable Applications for anti-NCOA3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:175000), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately -148 kD in size corresponding to NCOA3 by western blotting in t...
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
• Immunogen Type: Synthetic peptide
• Immunogen: NCOA3 / SRC-3 / AIB1 antibody was raised against synthetic peptide corresponding to an internal region of human NCOA3 isoform a protein.
• Antigen Modification: Internal
• Preparation and Storage: Long term: -20 degree C; Short term: -20 degree C

Immunohistochemistry (IHC)

(Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-NCOA3 / SRC-3 / AIB1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Anti-NCOA3 Antibody - Western Blot. Western blot of affinity purified anti-NCOA3 antibody shows detection of NCOA3 in mouse liver nuclear extract (lane 1), transient transfected 293 cell lysate (lane 2), HeLa whole cell lysate (lane 3) and mouse thyroid cell nuclear extract (lane 4). The band at ~148 kD, indicated by the arrowhead, corresponds to NCOA3. Mouse NCOA3 (lanes 1 and 4) appear as ~105 kD bands. Pre-incubation of antibody with the immunizing peptide blocks detection of specific band staining (not shown). Personal communication, H. Ying, NCI, Bethesda, MD.)

Related Product Information for anti-NCOA3 antibody

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation.

NCBI and Uniprot Product Information

• NCBI GI #: 32307124
• NCBI GeneID: 8202
• NCBI Accession #: NP_006525.2
• NCBI GenBank Nucleotide #: NM_006534.3
• UniProt Accession #: Q9Y6Q9; Q0VF45; Q5JYD9; Q5JYE0; Q9BR49; Q9UPC9; Q9UPG4; Q9UPG7; A4LAZ5
• Molecular Weight: 154,892 Da
• NCBI Official Full Name: nuclear receptor coactivator 3 isoform b
• NCBI Official Synonym Full Names: nuclear receptor coactivator 3
• NCBI Official Symbol: NCOA3
• NCBI Official Synonym Symbols: ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
• NCBI Protein Information: nuclear receptor coactivator 3; CBP-interacting protein; receptor-associated coactivator 3; amplified in breast cancer 1 protein; steroid receptor coactivator protein 3; class E basic helix-loop-helix protein 42; thyroid hormone receptor activator molecule 1
• UniProt Protein Name: Nuclear receptor coactivator 3
• Protein Family: Nuclear receptor coactivator
• UniProt Gene Name: NCOA3
• UniProt Synonym Gene Names: AIB1; BHLHE42; RAC3; TRAM1; NCoA-3; AIB-1; pCIP; bHLHe42; RAC-3; SRC-3; TRAM-1
• UniProt Entry Name: NCOA3_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]

Uniprot Description

SRC-3: a histone acetyltransferase and nuclear receptor coactivator. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Activity is enhanced by various hormones and TNF. Acetylation by CREBBP may suppress coactivation of target genes by disrupting its association with nuclear receptors. Frequently amplified or overexpressed in breast and ovarian cancers. Five alternatively spliced isoforms have been described.

Protein type: Acetyltransferase; Transcription, coactivator/corepressor; EC 2.3.1.48; Motility/polarity/chemotaxis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20q12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein dimerization activity; ligand-dependent nuclear receptor binding; protein binding; signal transducer activity; histone acetyltransferase activity; ligand-dependent nuclear receptor transcription coactivator activity; androgen receptor binding; transcription coactivator activity; protein N-terminus binding; thyroid hormone receptor binding; chromatin binding; nuclear hormone receptor binding

Biological Process: developmental growth; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; multicellular organism growth; androgen receptor signaling pathway; vagina development; positive regulation of transcription from RNA polymerase II promoter; histone acetylation; cellular response to hormone stimulus; positive regulation of keratinocyte differentiation