Western Blot (WB)
(NCOA3 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NCOA3 antibody detected the NCOA3 protein (arrow).)
Rabbit anti-Human NCOA3 Polyclonal Antibody | anti-NCOA3 antibody
NCOA3, NT (NCOA3, AIB1, BHLHE42, RAC3, TRAM1, Nuclear receptor coactivator 3, ACTR, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42, Receptor-associated coactivator 3, Steroid receptor coactivator p
Gene Names
NCOA3; ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NCOA3; Polyclonal Antibody; NT (NCOA3; AIB1; BHLHE42; RAC3; TRAM1; Nuclear receptor coactivator 3; ACTR; Amplified in breast cancer 1 protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; Receptor-associated coactivator 3; Steroid receptor coactivator p; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; anti-NCOA3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-NCOA3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
NCOA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 344-372 amino acids from the N-terminal region of human NCOA3.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(NCOA3 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NCOA3 antibody detected the NCOA3 protein (arrow).)
Western Blot (WB)
(NCOA3 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NCOA3 antibody detected the NCOA3 protein (arrow).)
Related Product Information for anti-NCOA3 antibody
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
Product Categories/Family for anti-NCOA3 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Cai, D., et al. Cancer Res. 70(16):6477-6485(2010). Kovanen, L., et al. Alcohol Alcohol. 45(4):303-311(2010). Karmakar, S., et al. Mol. Endocrinol. 24(6):1187-1202(2010). Kleibl, Z., et al. J. Cancer Res. Clin. Oncol. (2010).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
154,892 Da
NCBI Official Full Name
nuclear receptor coactivator 3 isoform d
NCBI Official Synonym Full Names
nuclear receptor coactivator 3
NCBI Official Symbol
NCOA3
NCBI Official Synonym Symbols
ACTR; AIB1; RAC3; SRC3; pCIP; AIB-1; CTG26; SRC-3; CAGH16; KAT13B; TNRC14; TNRC16; TRAM-1; bHLHe42
NCBI Protein Information
nuclear receptor coactivator 3; CBP-interacting protein; amplified in breast cancer 1 protein; class E basic helix-loop-helix protein 42; receptor-associated coactivator 3; steroid receptor coactivator protein 3; thyroid hormone receptor activator molecul
UniProt Protein Name
Nuclear receptor coactivator 3
Protein Family
UniProt Gene Name
NCOA3
UniProt Synonym Gene Names
AIB1; BHLHE42; RAC3; TRAM1; NCoA-3; AIB-1; pCIP; bHLHe42; RAC-3; SRC-3; TRAM-1
UniProt Entry Name
NCOA3_HUMAN
NCBI Description
The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Several transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein. [provided by RefSeq, Mar 2010]
Research Articles on NCOA3
Similar Products
Product Notes
The NCOA3 ncoa3 (Catalog #AAA6324190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA3, NT (NCOA3, AIB1, BHLHE42, RAC3, TRAM1, Nuclear receptor coactivator 3, ACTR, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42, Receptor-associated coactivator 3, Steroid receptor coactivator p reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NCOA3 ncoa3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
