Rabbit anti-Human EDA2R / XEDAR Polyclonal Antibody | anti-EDA2R antibody

Rabbit Polyclonal (IgG) to Human EDA2R / XEDAR

Cat. Num. AAA246927

• Gene Names: EDA2R; XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: EDA2R / XEDAR; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human EDA2R / XEDAR; Anti-EDA2R / XEDAR Antibody IHC-plus; EDA2R; EDA-A2R; EDAA2R; Ectodysplasin A2 receptor; TNFRSF27; XEDAR; EDA-A2 receptor; EDAR2; Human EDA2R; anti-EDA2R antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects human X-linked ectodermal dysplasia receptor (XEDAR), a 33kD type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily. XEDAR is a homologue of the ectodermal dysplasia receptor (EDAR), and binds to an isoform of ectodysplasin termed EDA-A2. Binding of ectodysplasin to XEDAR results in the induction of apoptosis, and is thought to be involved ectodermal organ development. New evidence suggests XEDAR may also be involved in skeletal muscle development. Two predominant isoforms of XEDAR exist; XEDAR-s and XEDAR-L, produced by alternative splicing.
• Purity/Purification: Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 297

• Applicable Applications for anti-EDA2R antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (2.5 ug/ml), WB (0.5 - 2 ug/ml); Usage: Western Blot: Detects a band of approximately 33kD in 293 cell lysates.
• Target Species: Human
• Immunogen Description: Recombinant human XEDAR.
• Immunogen Type: Recombinant protein
• Immunogen: EDA2R / XEDAR antibody was raised against recombinant human XEDAR.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-EDA2R antibody IHC of human testis, Leydig cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Related Product Information for anti-EDA2R antibody

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform.

NCBI and Uniprot Product Information

• NCBI GI #: 11140823
• NCBI GeneID: 60401
• NCBI Accession #: NP_068555.1
• NCBI GenBank Nucleotide #: NM_021783.3
• UniProt Accession #: Q9HAV5; Q5VYX9; Q5VYY0; Q6UWM2; Q8IZA6
• Molecular Weight: 32,959 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 27 isoform 1
• NCBI Official Synonym Full Names: ectodysplasin A2 receptor
• NCBI Official Symbol: EDA2R
• NCBI Official Synonym Symbols: XEDAR; EDAA2R; EDA-A2R; TNFRSF27
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 27; EDA-A2 receptor; X-linked ectodysplasin-A2 receptor; tumor necrosis factor receptor superfamily member XEDAR
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 27
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: EDA2R
• UniProt Synonym Gene Names: TNFRSF27; XEDAR; EDA-A2 receptor
• UniProt Entry Name: TNR27_HUMAN

NCBI Description

EDA-A1 and EDA-A2 are two isoforms of ectodysplasin that are encoded by the anhidrotic ectodermal dysplasia (EDA) gene. Mutations in EDA give rise to a clinical syndrome characterized by loss of hair, sweat glands, and teeth. The protein encoded by this gene specifically binds to EDA-A2 isoform. This protein is a type III transmembrane protein of the TNFR (tumor necrosis factor receptor) superfamily, and contains 3 cysteine-rich repeats and a single transmembrane domain but lacks an N-terminal signal peptide. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

EDA2R: Receptor for EDA isoform A2, but not for EDA isoform A1. Mediates the activation of the NF-kappa-B and JNK pathways. Activation seems to be mediated by binding to TRAF3 and TRAF6. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq12

Cellular Component: integral to plasma membrane; integral to membrane

Molecular Function: protein binding; tumor necrosis factor receptor activity; receptor activity

Biological Process: epidermis development; tumor necrosis factor-mediated signaling pathway; multicellular organismal development; positive regulation of JNK cascade; activation of NF-kappaB transcription factor