Rabbit anti-Human DARC Polyclonal Antibody | anti-DARC antibody

Rabbit Polyclonal (IgG) to Human DARC

Cat. Num. AAA246756

• Gene Names: ACKR1; FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DARC; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human DARC; Anti-DARC Antibody (aa1-50) IHC-plus; CD234; CD234 antigen; Duffy antigen; Duffy blood group; Duffy group antigen; Dfy; FY; Fy glycoprotein; Plasmodium vivax receptor; WBCQ1; CCBP1; Duffy blood group antigen; Glycoprotein D; GPD; GpFy; Human DARC; anti-DARC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD234 Antibody detects endogenous levels of total CD234 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 336

• Applicable Applications for anti-DARC antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: ELISA (1:40000), IF (1:100 - 1:500), IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human DARC (aa1-50).
• Immunogen: DARC antibody was raised against synthetic peptide from human DARC (aa1-50).
• Antigen Modification: aa1-50
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-DARC antibody IHC of human thymus, vessels. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of A549 cells, using CD234 Antibody. The picture on the right is treated with the synthesized peptide.)

Related Product Information for anti-DARC antibody

Duffy blood group antigens, part of the Chemokine Receptor family, appear to be multimeric erythrocyte-membrane protein receptors composed of different subunits. A glycoprotein, GPD, is the major subunit of the protein complex. Other components include the Rh, PGM1, Fy, and PEPC proteins. Duffy antigen is expressed by red blood cells, by postcapillary venule endothelium throughout the body, except in the liver, and by Purkinje cells of the cerebellum.

Product Categories/Family for anti-DARC antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 42822887
• NCBI GeneID: 2532
• NCBI Accession #: NP_002027.2
• NCBI GenBank Nucleotide #: NM_002036.3
• UniProt Accession #: Q16570; O75898; Q16300; Q8WWE3; Q9UJP0; Q9UKZ5; Q9UKZ6; Q9UQE1; A8YPG5
• Molecular Weight: 35,678 Da
• NCBI Official Full Name: atypical chemokine receptor 1 isoform b
• NCBI Official Synonym Full Names: atypical chemokine receptor 1 (Duffy blood group)
• NCBI Official Symbol: ACKR1
• NCBI Official Synonym Symbols: FY; Dfy; GPD; DARC; GpFy; CCBP1; CD234; WBCQ1
• NCBI Protein Information: atypical chemokine receptor 1; glycoprotein D; Fy glycoprotein; Duffy blood group antigen; plasmodium vivax receptor; Duffy blood group, chemokine receptor; Duffy blood group, atypical chemokine receptor
• UniProt Protein Name: Atypical chemokine receptor 1
• Protein Family: Darcynin
• UniProt Gene Name: ACKR1
• UniProt Synonym Gene Names: DARC; FY; GPD; GpFy
• UniProt Entry Name: ACKR1_HUMAN

NCBI Description

The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DARC: Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. Belongs to the G-protein coupled receptor Duffy family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, Duffy family; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: recycling endosome; early endosome; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; transmembrane receptor activity; C-C chemokine binding; receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; defense response; inflammatory response; regulation of chemokine production

Disease: White Blood Cell Count Quantitative Trait Locus 1; Blood Group, Duffy System; Malaria, Susceptibility To