Mouse anti-Human ATP6V1D / V-ATPase subunit D Monoclonal Antibody | anti-ATP6V1D antibody

Mouse Monoclonal [clone 3G4] (IgG2a,k) to Human ATP6V1D / V-ATPase subunit D

Cat. Num. AAA246552

• Gene Names: ATP6V1D; VATD; VMA8; ATP6M
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ATP6V1D / V-ATPase subunit D; Monoclonal Antibody; Mouse Monoclonal [clone 3G4] (IgG2a; k) to Human ATP6V1D / V-ATPase subunit D; Anti-ATP6V1D / V-ATPase subunit D Antibody (clone 3G4) IHC-plus; ATP6V1D; ATP6M; V-ATPase subunit D; VPATPD; V-type proton ATPase subunit D; Vacuolar H-ATPase subunit D; Vacuolar proton pump D subunit; Vacuolar proton pump subunit D; VMA8; V-ATPase d subunit; Vacuolar ATPase subunit D; VATD; Human ATP6V1D; anti-ATP6V1D antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3G4
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 247

• Applicable Applications for anti-ATP6V1D antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (2.5 ug/ml), WB; Usage: Tested in Western blot using the recombinant protein immunogen as the protein source.
• Target Species: Human
• Immunogen Description: ATP6V1D (NP_057078, 69 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: ATP6V1D / V-ATPase subunit D antibody was raised against aTP6V1D (NP_057078, 69 a.a. ~ 169 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATP6V1D antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ATP6V1D antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Product Categories/Family for anti-ATP6V1D antibody

Family: Transporter; Subfamily: ATPase - V type

NCBI and Uniprot Product Information

• NCBI GI #: 7706757
• NCBI GeneID: 51382
• NCBI Accession #: NP_057078.1
• NCBI GenBank Nucleotide #: NM_015994.3
• UniProt Accession #: Q9Y5K8; Q9Y688; B2RE33
• Molecular Weight: 28,263 Da
• NCBI Official Full Name: V-type proton ATPase subunit D
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• NCBI Official Symbol: ATP6V1D
• NCBI Official Synonym Symbols: VATD; VMA8; ATP6M
• NCBI Protein Information: V-type proton ATPase subunit D; V-ATPase D subunit; V-ATPase subunit D; vacuolar H-ATPase subunit D; vacuolar proton pump D subunit; vacuolar proton pump subunit D; vacuolar ATP synthase subunit D; vacuolar proton-ATPase subunit D; V-ATPase 28 kDa accessory protein; vacuolar proton pump delta polypeptide; ATPase, H+ transporting lysosomal, member M; H(+)-transporting two-sector ATPase, subunit M; ATPase, H+ transporting, lysosomal (vacuolar proton pump)
• UniProt Protein Name: V-type proton ATPase subunit D
• UniProt Gene Name: ATP6V1D
• UniProt Synonym Gene Names: ATP6M; VATD; V-ATPase subunit D
• UniProt Entry Name: VATD_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1D: Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Belongs to the V-ATPase D subunit family.

Protein type: Energy Metabolism - oxidative phosphorylation; Hydrolase; EC 3.6.3.14

Chromosomal Location of Human Ortholog: 14q23-q24.2

Cellular Component: centrosome; membrane; lysosomal membrane; cytosol; cilium

Molecular Function: protein binding; ATPase activity, coupled to transmembrane movement of substances

Biological Process: interaction with host; proton transport; cellular iron ion homeostasis; insulin receptor signaling pathway; cilium biogenesis; transferrin transport; transmembrane transport