anti-Human SCP3 Polyclonal Antibody | anti-SCP3 antibody

Anti-SCP3 Antibody

Cat. Num. AAA177709

• Gene Names: SYCP3; COR1; SCP3; SPGF4; RPRGL4
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: SCP3; Polyclonal Antibody; Anti-SCP3 Antibody; Synaptonemal complex protein 3; choline phosphotransferase 1; chpt1; COR 1; COR1; MGC71888; RNASCP3; SCP 3; SCP-3; SPGF4; Sycp 3; Sycp3; SYCP3_HUMAN; synaptonemal complex protein 3; anti-SCP3 antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 236

• Applicable Applications for anti-SCP3 antibody: Western Blot (WB)
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml
• Immunogen: E Coli-derived human SCP3 recombinant protein (Position: E18-E223). Human SCP3 shares 72% and 73% amino acid (aa) sequences identity with mouse and rat SCP3, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-SCP3 Picoband antibody, -1.jpgAll lanes: Anti SCP3 at 0.5ug/mlWB: Recombinant Human SCP3 Protein 0.5ngPredicted bind size: 41KDObserved bind size: 41KD)

Western Blot (WB)

(Anti-SCP3 Picoband antibody, -2.jpgAll lanes: Anti SCP3 at 0.5ug/mlWB: HT1080 Whole Cell Lysate at 40ugPredicted bind size: 28KDObserved bind size: 28KD)

Related Product Information for anti-SCP3 antibody

Description: Rabbit IgG polyclonal antibody for Synaptonemal complex protein 3(SYCP3) detection. Tested with WB in Human.

Background: SYCP3, also known as COR1 or SCP3, is a protein that in humans is encoded by the SYCP3 gene. It is mapped to 12q23.2. This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. SYCP3 has an essential meiotic function in human spermatogenesis that is compromised by the mutant protein by dominant-negative interference. SYCP3 is linked to inherited aneuploidy in female germ cells and provides a model system for studying age-dependent degeneration in oocytes.

References

1. Martinez-Garay I, Jablonka S, Sutajova M, Steuernagel P, Gal A, Kutsche K (Sep 2002). "A new gene family (FAM9) of low-copy repeats in Xp22.3 expressed exclusively in testis: implications for recombinations in this region".Genomics 80 (3): 259-67. 2. Mallon AM, Platzer M, Bate R, Gloeckner G, Botcherby MR, Nordsiek G, Strivens MA, Kioschis P, Dangel A, Cunningham D, Straw RN, Weston P, Gilbert M, Fernando S, Goodall K, Hunter G, Greystrong JS, Clarke D, Kimberley C, Goerdes M, Blechschmidt K, Rump A, Hinzmann B, Mundy CR, Miller W, Poustka A, Herman GE, Rhodes M, Denny P, Rosenthal A, Brown SD (Aug 2000). "Comparative genome sequence analysis of the Bpa/Str region in mouse and Man". Genome Res 10 (6): 758-75. 3. Miyamoto, T., Hasuike, S., Yogev, L., Maduro, M. R., Ishikawa, M., Westphal, H., Lamb, D. J. Azoospermia in patients heterozygous for a mutation in SYCP3. Lancet 362: 1714-1719, 2003.

NCBI and Uniprot Product Information

• NCBI GI #: 295789050
• NCBI GeneID: 50511
• NCBI Accession #: NP_001171419.1
• NCBI GenBank Nucleotide #: NM_001177948.1
• UniProt Accession #: Q8IZU3
• Molecular Weight: 27,729 Da
• NCBI Official Full Name: synaptonemal complex protein 3
• NCBI Official Synonym Full Names: synaptonemal complex protein 3
• NCBI Official Symbol: SYCP3
• NCBI Official Synonym Symbols: COR1; SCP3; SPGF4; RPRGL4
• NCBI Protein Information: synaptonemal complex protein 3
• UniProt Protein Name: Synaptonemal complex protein 3
• UniProt Gene Name: SYCP3
• UniProt Synonym Gene Names: SCP3; SCP-3
• UniProt Entry Name: SYCP3_HUMAN

NCBI Description

This gene encodes an essential structural component of the synaptonemal complex. This complex is involved in synapsis, recombination and segregation of meiotic chromosomes. Mutations in this gene are associated with azoospermia in males and susceptibility to pregnancy loss in females. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2010]

Uniprot Description

SYCP3: Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. Defects in SYCP3 are the cause of spermatogenic failure type 4 (SPGF4). An infertility disorder characterized by azoospermia, a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. Belongs to the XLR/SYCP3 family.

Chromosomal Location of Human Ortholog: 12q

Cellular Component: chromosome, pericentric region; lateral element; nucleus

Molecular Function: DNA binding

Biological Process: cell division; male meiosis I; spermatogenesis, exchange of chromosomal proteins

Disease: Spermatogenic Failure 4