Western Blot (WB)
(SIRT4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SIRT4 antibody detected the SIRT4 protein (arrow).)
Rabbit anti-Human, Mouse SIRT4 Polyclonal Antibody | anti-Sirt4 antibody
SIRT4, NT (SIRT4, SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4)
Gene Names
Sirt4; CG3187; D.mel3; DmelCG3187; dmSRT407; dSIRT4
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SIRT4; Polyclonal Antibody; NT (SIRT4; SIR2L4; NAD-dependent protein deacetylase sirtuin-4; NAD-dependent ADP-ribosyltransferase sirtuin-4; Regulatory protein SIR2 homolog 4; SIR2-like protein 4); Anti -SIRT4; anti-Sirt4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Sirt4 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
SIRT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-95 amino acids from the N-terminal region of human SIRT4.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(SIRT4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SIRT4 antibody detected the SIRT4 protein (arrow).)
Western Blot (WB)
(SIRT4 Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SIRT4 antibody detected the SIRT4 protein (arrow).)
Related Product Information for anti-Sirt4 antibody
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.
Product Categories/Family for anti-Sirt4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,991 Da
NCBI Official Full Name
Sirt4, isoform C
NCBI Official Symbol
Sirt4
NCBI Official Synonym Symbols
CG3187; D.mel3; DmelCG3187; dmSRT407; dSIRT4
NCBI Protein Information
CG3187 gene product from transcript CG3187-RC; CG3187-PA; CG3187-PB; CG3187-PC; Sirt4; Sirt4-PA; Sirt4-PB; Sirt4-PC
UniProt Protein Name
NAD-dependent protein deacetylase Sirt4
Protein Family
UniProt Gene Name
Sirt4
UniProt Entry Name
SIR4_DROME
Uniprot Description
Function: NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form
By similarity. HAMAP-Rule MF_03161
Catalytic activity: NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. HAMAP-Rule MF_03161
Cofactor: Binds 1 zinc ion per subunit
By similarity. HAMAP-Rule MF_03161
Subcellular location: Mitochondrion matrix
By similarity HAMAP-Rule MF_03161.
Sequence similarities: Belongs to the sirtuin family. Class II subfamily.Contains 1 deacetylase sirtuin-type domain.
Similar Products
Product Notes
The Sirt4 sirt4 (Catalog #AAA6011228) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT4, NT (SIRT4, SIR2L4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SIRT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the Sirt4 sirt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIRT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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