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Immunohistochemistry (IHC) (Anti-LRRFIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Rabbit anti-Human TRIP / LRRFIP1 Polyclonal Antibody | anti-TRIP antibody

Rabbit Polyclonal to Human TRIP / LRRFIP1

Gene Names
LRRFIP1; GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity Purified
Synonyms
TRIP / LRRFIP1; Polyclonal Antibody; Rabbit Polyclonal to Human TRIP / LRRFIP1; Anti-TRIP / LRRFIP1 Antibody (C-Terminus) IHC-plus; LRRFIP1; FLAP-1; FLAP1; FLIIAP1; GCF2; LRR FLII-interacting protein 1; HUFI-1; Tcf9; Tcf-9; TRIP; NEDD8-conjugating enzyme; TAR RNA-interacting protein; Transcription factor 9; GC-binding factor 2; GCF-2; Human TRIP; anti-TRIP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human LRRFIP1. LRRFIP1 antibody is predicted to not cross-react with other LRRFIP family members. Three isoforms of LRRFIP1 are known to exist; this antibody will only recognize the two longer isoforms.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, 0.02% sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
784
Applicable Applications for anti-TRIP antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
Application Notes
IHC-P (5 ug/ml), WB (1 - 2 ug/ml)
Target Species
Human
Immunogen Description
A 19 amino acid peptide near the carboxy terminus of human LRRFIP1
Immunogen Type
Synthetic peptide
Antigen Modification
C-Terminus
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-LRRFIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC) (Anti-LRRFIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 ug/ml.)

Immunofluorescence (IF) (Immunofluorescence of LRRFIP1 in human colon tissue with LRRFIP1 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 ug/ml.)

Western Blot (WB) (Western blot analysis of LRRFIP1 in human colon tissue lysate with LRRFIP1 antibody at 1 ug/ml.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,060 Da
NCBI Official Full Name
leucine-rich repeat flightless-interacting protein 1 isoform 4
NCBI Official Synonym Full Names
leucine rich repeat (in FLII) interacting protein 1
NCBI Official Symbol
LRRFIP1
NCBI Official Synonym Symbols
GCF2; TRIP; FLAP1; GCF-2; FLAP-1; HUFI-1; FLIIAP1
NCBI Protein Information
leucine-rich repeat flightless-interacting protein 1; GC-binding factor 2; NEDD8-conjugating enzyme; TAR RNA-interacting protein; LRR FLII-interacting protein 1
UniProt Protein Name
Leucine-rich repeat flightless-interacting protein 1
UniProt Gene Name
LRRFIP1
UniProt Synonym Gene Names
GCF2; TRIP; LRR FLII-interacting protein 1
UniProt Entry Name
LRRF1_HUMAN

Uniprot Description

LRRFIP1: Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding. Belongs to the LRRFIP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; RNA-binding

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; DNA binding; double-stranded RNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of interferon type I production; innate immune response; negative regulation of transcription, DNA-dependent

Research Articles on TRIP

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Product Notes

The TRIP lrrfip1 (Catalog #AAA244514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human TRIP / LRRFIP1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIP / LRRFIP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB). IHC-P (5 ug/ml), WB (1 - 2 ug/ml). Researchers should empirically determine the suitability of the TRIP lrrfip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIP / LRRFIP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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