Goat ALOX5AP / FLAP Polyclonal Antibody | anti-ALOX5AP antibody

Goat Polyclonal to Human ALOX5AP / FLAP

Cat. Num. AAA244334

• Gene Names: ALOX5AP; FLAP
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Bat, Dog, Horse (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ALOX5AP / FLAP; Polyclonal Antibody; Goat Polyclonal to Human ALOX5AP / FLAP; Anti-ALOX5AP / FLAP Antibody (C-Terminus) IHC-plus; ALOX5AP; FLAP; MK-886-binding protein; Human ALOX5AP; anti-ALOX5AP antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Gibbon, Monkey, Bat, Dog, Horse (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human ALOX5AP / FLAP.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 161

• Applicable Applications for anti-ALOX5AP antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (3.75 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KTISTTISPLLLIP from the C-terminus of human ALOX5AP / FLAP (NP_001620.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Marmoset, Dog, Bat, Horse, Opossum (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: ALOX5AP / FLAP antibody was raised against synthetic peptide C-KTISTTISPLLLIP from the C-terminus of human ALOX5AP / FLAP (NP_001620.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Marmoset, Dog, Bat, Horse, Opossum (93%); Monkey, Mouse, Rat, Elephant, Bovine, Pig, Platypus, Lizard (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ALOX5AP / FLAP antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ALOX5AP / FLAP antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ALOX5AP / FLAP antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 15718675
• NCBI GeneID: 241
• NCBI Accession #: NP_001620.2
• NCBI GenBank Nucleotide #: NM_001629.3
• UniProt Accession #: P20292; Q5VV04
• Molecular Weight: 18,157 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase-activating protein
• NCBI Official Symbol: ALOX5AP
• NCBI Official Synonym Symbols: FLAP
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein; MK-886-binding protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Arachidonate 5-lipoxygenase-activating protein
• UniProt Gene Name: ALOX5AP
• UniProt Synonym Gene Names: FLAP
• UniProt Entry Name: AL5AP_HUMAN

NCBI Description

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.

Protein type: Nuclear envelope; Membrane protein, integral; Activator; Membrane protein, multi-pass; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope

Molecular Function: arachidonic acid binding; protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; protein N-terminus binding; glutathione peroxidase activity

Biological Process: leukotriene biosynthetic process; positive regulation of catalytic activity; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Stroke, Ischemic