FLAP Recombinant Protein | ALOX5AP recombinant protein

FLAP Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120884

• Gene Names: ALOX5AP; FLAP
• Purity: >90% as determined by SDS-PAGE
• Synonyms: FLAP; FLAP Protein; Human; Recombinant (His Tag); Human ALOX5AP/FLAP Protein (His Tag); arachidonate 5-lipoxygenase-activating protein; ALOX5AP recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% gly, pH7.4, 0.5% triton, 5mM DTT. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of
• Sequence: Met1-Pro161

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of human ALOX5AP (NP_001620.2)(Met 1-Pro 161) was fused with a polyhistidine tag at the carboxy-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ALOX5AP recombinant protein

Background: Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP), also known as FLAP, belongs to the MAPEG family. ALOX5AP/FLAP is an essential partner of 5-LO for this process. The FLAP (ALOX5AP) gene has been linked to risk for myocardial infarction, stroke and restenosis, reigniting pharmaceutical interest in this target. It had been found that ALOX5AP/FLAP is a key enzyme in leukotriene formation, in both human pulmonary microvascular endothelial cells and a transformed human brain endothelial cell line. In addition, the protein FLAP has recently been identified as an emerging target in metabolic disease. In fact, FLAP is overexpressed in the adipose tissue of patients and experimental animals with obesity.

References

Gonsalves CS, et al. (2010) Hypoxia-mediated expression of 5-lipoxygenase-activating protein involves HIF-1alpha and NF-kappaB and microRNAs 135a and 199a-5p. J Immunol. 184(7): 3878-88.Zintzaras E, et al. (2009) Variants of the arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene and risk of stroke: a HuGE gene-disease association review and meta-analysis. Am J Epidemiol. 169(5): 523-32.Evans JF, et al. (2008) What's all the FLAP about: 5-lipoxygenase-activating protein inhibitors for inflammatory diseases. Trends Pharmacol Sci. 29(2): 72-8.Bck M, et al. (2007) 5-Lipoxygenase-activating protein: a potential link between innate and adaptive immunity in atherosclerosis and adipose tissue inflammation. Circ Res. 100: 946-9.

NCBI and Uniprot Product Information

• NCBI GI #: 15718675
• NCBI GeneID: 241
• NCBI Accession #: NP_001620.2
• NCBI GenBank Nucleotide #: NM_001629.3
• UniProt Accession #: P20292; Q5VV04
• Molecular Weight: 18,157 Da
• NCBI Official Full Name: arachidonate 5-lipoxygenase-activating protein isoform 1
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase-activating protein
• NCBI Official Symbol: ALOX5AP
• NCBI Official Synonym Symbols: FLAP
• NCBI Protein Information: arachidonate 5-lipoxygenase-activating protein; MK-886-binding protein
• UniProt Protein Name: Arachidonate 5-lipoxygenase-activating protein
• Protein Family: Flap endonuclease
• UniProt Gene Name: ALOX5AP
• UniProt Synonym Gene Names: FLAP
• UniProt Entry Name: AL5AP_HUMAN

NCBI Description

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]