Rabbit anti-Human MAEA Polyclonal Antibody | anti-MAEA antibody

MAEA, NT (MAEA, EMP, Macrophage erythroblast attacher, Cell proliferation-inducing gene 5 protein, Erythroblast macrophage protein, Human lung cancer oncogene 10 protein) (Biotin)

Cat. Num. AAA6317925

• Gene Names: MAEA; EMP; EMLP; GID9; PIG5; HLC-10
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAEA; Polyclonal Antibody; NT (MAEA; EMP; Macrophage erythroblast attacher; Cell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; Human lung cancer oncogene 10 protein) (Biotin); Human lung cancer oncogene 10 protein; anti-MAEA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAEA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the N-terminal region of human MAEA.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAEA Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the MAEA antibody detected the MAEA protein (arrow).)

Related Product Information for anti-MAEA antibody

This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Product Categories/Family for anti-MAEA antibody

Antibodies; Cancer Markers

References

Kobayashi, N., et al. Gene 396(2):236-247(2007). Bala, S., et al. Biochem. Biophys. Res. Commun. 342(4):1040-1048(2006). Hanspal, M., et al. Blood 92(8):2940-2950(1998).

NCBI and Uniprot Product Information

• NCBI GI #: 62953131
• NCBI GeneID: 10296
• NCBI Accession #: NP_001017405.1; NP_005873.2
• NCBI GenBank Nucleotide #: NM_001017405.1
• UniProt Accession #: Q7L5Y9; O95285; Q5JB54; Q6ZRD6; Q9BQ11; Q9H9V6; Q9H9Z4; Q9NW84
• Molecular Weight: 45,287 Da
• NCBI Official Full Name: macrophage erythroblast attacher isoform 1
• NCBI Official Synonym Full Names: macrophage erythroblast attacher
• NCBI Official Symbol: MAEA
• NCBI Official Synonym Symbols: EMP; EMLP; GID9; PIG5; HLC-10
• NCBI Protein Information: macrophage erythroblast attacher; proliferation-inducing gene 5; lung cancer-related protein 10; erythroblast macrophage protein; GID complex subunit 9, FYV10 homolog; human lung cancer oncogene 10 protein; cell proliferation-inducing gene 5 protein
• UniProt Protein Name: Macrophage erythroblast attacher
• Protein Family: Macrophage erythroblast attacher
• UniProt Gene Name: MAEA
• UniProt Synonym Gene Names: EMP; HLC-10
• UniProt Entry Name: MAEA_HUMAN

NCBI Description

This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

MAEA: Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA- mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion; Cell development/differentiation; Actin-binding

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: nucleoplasm; cytoskeleton; nuclear matrix; integral to plasma membrane; cytoplasm; contractile ring; spindle; nucleus

Molecular Function: actin binding

Biological Process: negative regulation of myeloid cell apoptosis; erythrocyte maturation; cell division; regulation of mitotic cell cycle; enucleate erythrocyte development; cytoskeleton organization and biogenesis; cell adhesion; cell cycle