Rabbit SLC2A1 / GLUT-1 Polyclonal Antibody | anti-SLC2A1 antibody

Rabbit Polyclonal (IgG) to Human SLC2A1 / GLUT-1

Cat. Num. AAA242720

• Gene Names: SLC2A1; PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: SLC2A1 / GLUT-1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human SLC2A1 / GLUT-1; Anti-SLC2A1 / GLUT-1 Antibody (aa441-490) IHC-plus; SLC2A1; EIG12; GLUT1DS; DYT17; DYT18; DYT9; Glucose transporter 1; HepG2 glucose transporter; Glucose transporter GLUT1; GLUT-1; GLUT1; PED; Human SLC2A1; anti-SLC2A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GLUT1 Antibody detects endogenous levels of total GLUT1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 492

• Applicable Applications for anti-SLC2A1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:40000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human GLUT1 (aa441-490).
• Immunogen: SLC2A1 / GLUT-1 antibody was raised against synthetic peptide from human GLUT1 (aa441-490).
• Antigen Modification: aa441-490
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-SLC2A1 / GLUT1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from Jurkat cells, using GLUT1 Antibody. The lane on the right is treated with the synthesized peptide.)

Product Categories/Family for anti-SLC2A1 antibody

Family: Transporter; Subfamily: Sugar transporter

NCBI and Uniprot Product Information

• NCBI GI #: 166795299
• NCBI GeneID: 6513
• NCBI Accession #: NP_006507.2
• NCBI GenBank Nucleotide #: NM_006516.2
• UniProt Accession #: P11166; O75535; Q147X2; A8K9S6; B2R620; D3DPX0
• Molecular Weight: 54,084 Da
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 1
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 1
• NCBI Official Symbol: SLC2A1
• NCBI Official Synonym Symbols: PED; DYT9; GLUT; DYT17; DYT18; EIG12; GLUT1; HTLVR; GLUT-1; GLUT1DS
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 1; hepG2 glucose transporter; receptor for HTLV-1 and HTLV-2; glucose transporter type 1, erythrocyte/brain; human T-cell leukemia virus (I and II) receptor
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 1
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC2A1
• UniProt Synonym Gene Names: GLUT1; GLUT-1
• UniProt Entry Name: GTR1_HUMAN

NCBI Description

This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]

Uniprot Description

GLUT1: an integral membrane protein that plays an important role in the glycolytic pathway by serving as a uniporter for glucose. One of 13 members of the human equilibrative glucose transport protein family. Transports a wide range of aldoses, including both pentoses and hexoses, and dehydroascorbic acid. Shown to transport water against an osmotic gradient. A receptor for the Human T-cell Leukemia virus (HTLV). Plays a role in the constitutive or basal uptake of glucose. Expressed at highest levels in proliferating cells of the early developing embryo, cells forming the blood tissue barriers, in human erythrocytes, astrocytes and in cardiac muscle. GLUT1 and GLUT3 are both essential for normal embryonic development. Is practically the only member of the GLUT family expressed on human red blood cells, where it comprises 10 - 20% of the integral membrane protein content. Several glycolytic proteins including the transporters GLUT1 and GLUT3, as well as multiple enzymes including HK2, PFKL, LDHA, ALDOA, ALDOC, PGK1, ENO1, PKM2, CA9 and PFKFB3 are induced in cancer cells by HIF-1 alpha. Polyps from Peutz-Jeghers patients exhibit up-regulated mTORC1 signaling, HIF-1alpha, and GLUT1 levels. Defects in GLUT1 are the cause of autosomal dominant GLUT1 deficiency syndrome, a blood-brain barrier glucose transport defect characterized by infantile seizures, delayed development, and acquired microcephaly. Defects also cause dystonia type 18, an exercise-induced paroxysmal dystonia/dyskinesia. Cytochalasin B binds to its inner surface, inhibiting its glucose transport activity with an IC50 of 0.44 uM.

Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; Transporter, SLC family

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: cortical actin cytoskeleton; membrane; basolateral plasma membrane; integral to plasma membrane; plasma membrane; melanosome; female pronucleus; intercellular junction; midbody; caveola; cytosol

Molecular Function: D-glucose transmembrane transporter activity; identical protein binding; xenobiotic transporter activity; protein binding; protein self-association; dehydroascorbic acid transporter activity; glucose transmembrane transporter activity; kinase binding

Biological Process: vitamin metabolic process; L-ascorbic acid metabolic process; pathogenesis; glucose transport; response to osmotic stress; cellular response to glucose starvation; hexose transport; carbohydrate metabolic process; energy reserve metabolic process; xenobiotic transport; protein complex assembly; regulation of insulin secretion; transmembrane transport; water-soluble vitamin metabolic process

Disease: Epilepsy, Idiopathic Generalized, Susceptibility To, 12; Dystonia 9; Glut1 Deficiency Syndrome 1; Glut1 Deficiency Syndrome 2