Goat TRIM2 Antibody | anti-TRIM2 antibody

TRIM2

Cat. Num. AAA555931

• Gene Names: TRIM2; CMT2R; RNF86
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: TRIM2; anti-TRIM2 antibody

• Host: Goat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Liquid
• Sequence Length: 771

• Applicable Applications for anti-TRIM2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the TRIM2 protein sequence according to NP_056086.2; NP_001123539.1

Immunohistochemistry (IHC)

(TRIM2 (dilution: 6.3 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Note cytoplasm staining in neuronal cell bodies. Protocol on datasheet.)

Immunohistochemistry (IHC)

(TRIM2 (dilution: 0.5 ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-TRIM2 antibody

TRIM RING finger protein TRIM2, a ubiquitin ligase, is highly expressed in the nervous system. It binds to neurofilament light subunit (NF-L);and regulates NF-L ubiquitination. Deficiencies in TRIM2 increases NF-L level in axons and NF-L-filled axonal swellings in cerebellum, retina, spinal cord, and cerebral cortex.

These deficiencies eventually lead to neurodegeneration, tremors and ataxia. It is proposed that TRIM2 has neuroprotection properties.

NCBI and Uniprot Product Information

• NCBI GI #: 194248079
• NCBI GeneID: 23321
• NCBI Accession #: NP_056086.2
• NCBI GenBank Nucleotide #: NM_015271.5
• UniProt Accession #: Q9C040
• NCBI Official Full Name: tripartite motif-containing protein 2 isoform 1
• NCBI Official Synonym Full Names: tripartite motif containing 2
• NCBI Official Symbol: TRIM2
• NCBI Official Synonym Symbols: CMT2R; RNF86
• NCBI Protein Information: tripartite motif-containing protein 2
• UniProt Protein Name: Tripartite motif-containing protein 2
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM2
• UniProt Synonym Gene Names: KIAA0517; RNF86
• UniProt Entry Name: TRIM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Uniprot Description

TRIM2: UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance. Belongs to the TRIM/RBCC family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ligase; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoplasm

Molecular Function: protein binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: regulation of neuron apoptosis; protein ubiquitination

Disease: Charcot-marie-tooth Disease, Axonal, Type 2r