Immunohistochemistry (IHC)
(Anti-Pan Cytokeratin antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse anti-Human Pan Cytokeratin Monoclonal Antibody
Anti-Pan Cytokeratin Antibody (clone C11)
IF: 1:50-1:200
IHC-P: 10 ug/ml
IP: 1:100
WB: 1:50-1:200
Long term: -20 degree C.
Avoid freeze-thaw cycles.
Immunohistochemistry (IHC)
(Anti-Pan Cytokeratin antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-Pan Cytokeratin antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy - Anti-Keratin monoclonal antibody. Immunofluorescence using Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 micron where the image is taken near the bottom of the cell. Use FITC a 1:2000 dilution of FITC conjugated Goat-a-Mouse IgG [H&L] ( for detection.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy - Anti-Keratin monoclonal antibody. Immunofluorescence using Mouse Anti-Keratin antibody. Confocal slices of HeLa cells are between 0.5 and 0.6 micron where the image is taken near the bottom of the cell. Use FITC a 1:2000 dilution of FITC conjugated Goat-a-Mouse IgG [H&L] ( for detection.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy- Anti-Keratin Antibody. Immunofluorescence Microscopy of Anti-Keratin antibody was used with Dylight 488 goat anti-mouse (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, polyclonal Anti-HDAC-1 antibody was used with Atto425 Anti-Rabbit IgG (shown in red) to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.)
Immunofluorescence (IF)
(Immunofluorescence Microscopy- Anti-Keratin Antibody. Immunofluorescence Microscopy of Anti-Keratin antibody was used with Dylight 488 goat anti-mouse (shown in green) to detect Keratin by Immunofluorescence. In the same experiment, polyclonal Anti-HDAC-1 antibody was used with Atto425 Anti-Rabbit IgG (shown in red) to detect HDAC-1. Data was collected on a STED-CW TCS-SP5 Confocal system (Leica Microsystems) equipped with a DFC 350FX camera allowing sequential acquisition in wide-field, confocal and STED CW imaging modes and provided courtesy of: Myriam Gastard, PhD, personal communication, Leica Microsystems, Inc. USA.)
Western Blot (WB)
(Western blot - Anti-Keratin Monoclonal Antibody. Western blot of Mouse Anti-Keratin antibody. This antibody recognizes a single 56 kD band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 ng of keratin from human epidermis (Sigma K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
Western Blot (WB)
(Western blot - Anti-Keratin Monoclonal Antibody. Western blot of Mouse Anti-Keratin antibody. This antibody recognizes a single 56 kD band corresponding to human keratin as confirmed by the position of molecular weight markers (not shown). Approximatley 100 ng of keratin from human epidermis (Sigma K0253) was applied under reducing conditions to a pre-cast 4-20% iGel from Gradipore Inc. A 1:400 dilution of Mab anti-Keratin was used for 2h followed by detection using a 1:5000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (and visualization using the Odyssey Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.)
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Product Notes
The Pan Cytokeratin (Catalog #AAA2400981) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Pan Cytokeratin Antibody (clone C11) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pan Cytokeratin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:500-1:20000 IF: 1:50-1:200 IHC-P: 10 ug/ml IP: 1:100 WB: 1:50-1:200. Researchers should empirically determine the suitability of the Pan Cytokeratin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pan Cytokeratin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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