CD79a/CD5 Dual Color Reagent | CD79a dual color reagent

MOUSE ANTI HUMAN CD79a:FITC/CD5:RPE

Cat. Num. AAA238077

• Gene Names: CD5; T1; LEU1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD79a/CD5; MOUSE ANTI HUMAN CD79a:FITC/CD5:RPE; CD79a dual color reagent

• Host: Mouse
• Isotype: FITC reagent: IgG1 (MOUSE) ; RPE reagent: IgG2a (MOUSE)
• Clone Number: ZL7-4/MF7-14.5
• Form/Format: FITC/RPE; Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
• Sequence Length: 495

• Applicable Applications for CD79a dual color reagent: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul to label 100ul of whole blood. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 0.5 ml distilled water; Perservative Stabilisers
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of normal human peripheral blood lymphocytes with MBS238077. Note differentiation of CD5 positive T cells (strong CD5), CD79a positive B cells and subset of CD5 positive B cells showing expression of CD79a and weak CD5 expression)

Related Product Information for CD79a dual color reagent

CD79a is part of the B cell receptor complex, and is expressed by all B lymphocytes. CD5 is a 67kD glycoprotein expressed by all mature T cells and by a subset of mature B cells. CD5 has been shown to act as a ligand for the B cell marker CD72. CD5 positive B cells have been implicated in the pathogenesis of various autoimmune disorders and are the normal counterpart of the malignant cells in B cell chronic lymphocytic leukaemia. This combination of antibodies is useful for simultaneously enumerating CD5 and CD79a positive cells. It may find particular application in the study of CD5 positive B lymphocytes and in the study of chronic lymphoproliferative diseases. The CD79a specific antibody in this combination has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma, see Bell, P.B. et al. for details.

NCBI and Uniprot Product Information

• NCBI GI #: 166197668
• NCBI GeneID: 921
• NCBI Accession #: NP_055022.2
• NCBI GenBank Nucleotide #: NM_014207.3
• Molecular Weight: 54,578 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Official Synonym Symbols: T1; LEU1
• NCBI Protein Information: T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: CD5
• UniProt Synonym Gene Names: LEU1
• UniProt Entry Name: CD5_HUMAN

Uniprot Description

CD5: a surface glycoprotein expressed on T cells and B-1 B cells. Rapidly recruited to the immunological synapse, lowering the response of the T cell antigen receptor. CD5 interacts with CD72/LYB-2. Promotes B-cell survival through stimulation of autocrine IL-10 production. Contains 3 SRCR domains.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; receptor activity; scavenger receptor activity

Biological Process: cell proliferation; receptor-mediated endocytosis; T cell costimulation; cell recognition