Testing Data
(FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat anti Mouse CD45R . All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Rat anti-Mouse CD45R Monoclonal Antibody | anti-CD45R antibody
Rat Anti Mouse CD45R: Amethyst Orange
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
This product contains a precipitate we recommend microcentrifugation before use.
Testing Data
(FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat anti Mouse CD45R . All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD19 and Amethyst Orange conjugated Rat anti Mouse CD45R . All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
Testing Data
(Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.Image caption:Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistochemistry. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F (2009)Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.PLoS ONE 4(12): e8291.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the enumeration of B cells in the intestinal mucosa by immunofluorescence.Image caption:Recruitment of inflammatory cells in the intestine of neonates after CpG-ODN treatment. Eight-day-old neonatal mice received 20 ug/g of CpG-ODN orally. Twenty-four hours after treatment, ilea were removed to analyze the recruitment of inflammatory cells by immunohistochemistry. Six to ten neonates from different litters (5 sections for each animal) per group were analyzed. Data were analyzed by non-parametric Mann-Whitney test.From: Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F (2009)Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation.PLoS ONE 4(12): e8291.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Figure A. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated rat anti mouse CD45R . All experiments performed on murine bone marrow in the presence of murine SeroBlock .)
Testing Data
(Figure A. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated hamster anti mouse CD79b and RPE conjugated rat anti mouse CD45R . All experiments performed on murine bone marrow in the presence of murine SeroBlock .)
Testing Data
(Figure A. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated rat anti mouse CD45 . All experiments performed on murine splenocytes in the presence of murine SeroBlock .)
Testing Data
(Figure A. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated mouse anti mouse CD28 and Alexa647 conjugated rat anti mouse CD45 . All experiments performed on murine splenocytes in the presence of murine SeroBlock .)
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat anti Mouse CD45R . All experiments were performed on mouse splenocytes in the presence of murine SeroBlock )
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat IgG2a isotype control . Figure B. RPE conjugated Rat anti Mouse CD36 and A488 conjugated Rat anti Mouse CD45R . All experiments were performed on mouse splenocytes in the presence of murine SeroBlock )
Testing Data
(Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the identification of marginal zone B cells in mouse spleen by immunofluorescence on cryostat sections.Image caption:Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou Z, Niu H, Zheng YY, Morel L.Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.BMC Immunol. 2011 Jan 20;12:7.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti Mouse CD45R antibody, clone RA3-6B2 used for the identification of marginal zone B cells in mouse spleen by immunofluorescence on cryostat sections.Image caption:Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou Z, Niu H, Zheng YY, Morel L.Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.BMC Immunol. 2011 Jan 20;12:7.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti MouseCD45R antibody, clone RA3-6B2 used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 ?m), (D) CD3 immunohistochemistry for T cells. Bars = 200 ?m.From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.PeerJ. 2016 Jan 26;4:e1600.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Testing Data
(Rat anti MouseCD45R antibody, clone RA3-6B2 used for the demonstration of B cells in murine spinal column by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.Image caption:Representative images of key immunohistochemical markers run on a longitudinally sectioned, decalcified spinal column from an EAE mouse with a clinical EAE score of 4.(A) HE, (B) B220 immunohistochemistry for (B) cells, (C) F4/80 immunohistochemistry for macrophages (arrow indicates area highlighted in inset, inset bar = 20 ?m), (D) CD3 immunohistochemistry for T cells. Bars = 200 ?m.From: Gibson-Corley KN, Boyden AW, Leidinger MR, Lambertz AM, Ofori-Amanfo G, Naumann PW, Goeken JA, Karandikar NJ.A method for histopathological study of the multifocal nature of spinal cord lesions in murine experimental autoimmune encephalomyelitis.PeerJ. 2016 Jan 26;4:e1600.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)
Rat anti Mouse CD45R antibody, clone RA3-6B2 is suitable for plp fixed paraffin embedded tissues (Whiteland et al.1995).
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Product Notes
The CD45R ptprc (Catalog #AAA225856) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Mouse CD45R: Amethyst Orange reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45R can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Min. Dilution: Neat Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B). Researchers should empirically determine the suitability of the CD45R ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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