Western Blot (WB)
(Western blot analysis of whole cell lysates probed with Mcl-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 2 second exposure. Arrow points to Mcl-1 (molecular weight 40 kDa))
Mouse Mcl-1 Monoclonal Antibody | anti-MCL1 antibody
Mcl-1 Antibody with Control Lysate
Gene Names
MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by affinity chromatography on Protein G from ascites
Synonyms
Mcl-1; Monoclonal Antibody; Mcl-1 Antibody with Control Lysate; Mouse anti Mcl-1; Mcl-1 Antibody; anti-MCL1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Purity/Purification
Purified by affinity chromatography on Protein G from ascites
Form/Format
Liquid in phosphate buffered saline with 0.09% sodium azide (NaN3).
Sequence Length
350
Applicable Applications for anti-MCL1 antibody
Western Blot (WB)
Application Notes
WB: 1:1000
Western Blotting: Detects a band of approximately 40 kDa in HeLa cell lysate.
Western Blotting: Detects a band of approximately 40 kDa in HeLa cell lysate.
Immunogen
E Coli derived recombinant protein corresponding to amino acids 1-230 of human Mcl-1
Lysate Composition
400ug HeLa lysate lyophilized in RIPA buffer.
Lysate Reconstitution
If using DDT reconstitute the lyophilized lysate with 190mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 10mul 2M DTT.
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
If using BME reconstitute the lyophilized lysate with 180mul DI H2O, add 200mul 2x Laemmli Sample Buffer and 20u
Preparation and Storage
Antibody: Store undiluted at -20 degree C, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Lysate: Store lyophilized lysate at -20 degree C.
After reconstitution aliquot and store at -20 degree C for up to 3 months or at -80 degree C for longer term storage. Shelf Life: As supplied, 12 months from date of despatch.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with Mcl-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 2 second exposure. Arrow points to Mcl-1 (molecular weight 40 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with Mcl-1 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000) and visualized on the ChemiDoc™ MP with 2 second exposure. Arrow points to Mcl-1 (molecular weight 40 kDa))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
40 kDa
NCBI Official Full Name
Induced myeloid leukemia cell differentiation protein Mcl-1
NCBI Official Synonym Full Names
MCL1, BCL2 family apoptosis regulator
NCBI Official Symbol
MCL1
NCBI Official Synonym Symbols
TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
NCBI Protein Information
induced myeloid leukemia cell differentiation protein Mcl-1
UniProt Protein Name
Induced myeloid leukemia cell differentiation protein Mcl-1
Protein Family
UniProt Gene Name
MCL1
UniProt Synonym Gene Names
BCL2L3; Bcl2-L-3
NCBI Description
This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]
Uniprot Description
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Research Articles on MCL1
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Product Notes
The MCL1 mcl1 (Catalog #AAA225494) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mcl-1 Antibody with Control Lysate reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mcl-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 Western Blotting: Detects a band of approximately 40 kDa in HeLa cell lysate. Researchers should empirically determine the suitability of the MCL1 mcl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mcl-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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