Sheep Bruton's Tyrosine Kinase ELISA Kit | Btk elisa kit

Sheep Bruton's Tyrosine Kinase ELISA Kit

Cat. Num. AAA743445

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• Reactivity: Sheep
• Synonyms: Bruton's Tyrosine Kinase; Sheep Bruton's Tyrosine Kinase ELISA Kit; Btk elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Btk ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Btk. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Btk elisa kit

Principle of the Assay: Btk ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Btk antibody and an Btk-HRP conjugate. The assay sample and buffer are incubated together with Btk-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Btk concentration since Btk from samples and Btk-HRP conjugate compete for the anti-Btk antibody binding site. Since the number of sites is limited, as more sites are occupied by Btk from the sample, fewer sites are left to bind Btk-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Btk concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Btk elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1684918
• NCBI GeneID: 695
• Molecular Weight: 79,937 Da
• NCBI Official Full Name: Bruton's tyrosine kinase
• NCBI Official Synonym Full Names: Bruton agammaglobulinemia tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK; B-cell progenitor kinase; agammaglobulinaemia tyrosine kinase; truncated Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK isoform (lacking exon 14); dominant-negative kinase-deficient Brutons tyrosine kinase
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK
• UniProt Entry Name: BTK_HUMAN

NCBI Description

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; protein-tyrosine kinase activity; metal ion binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; B cell activation; transcription, DNA-dependent; cell maturation; calcium-mediated signaling; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; regulation of cell proliferation; regulation of B cell cytokine production; regulation of B cell apoptosis; MyD88-dependent toll-like receptor signaling pathway; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; positive regulation of B cell differentiation; innate immune response; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked