Rabbit anti-Mouse, Rat TEM7 Polyclonal Antibody | anti-TEM7 antibody

RABBIT ANTI TEM7

Cat. Num. AAA223982

• Gene Names: PLXDC1; TEM3; TEM7
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: TEM7; Polyclonal Antibody; RABBIT ANTI TEM7; anti-TEM7 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human Tumour endothelial marker 7 (TEM7), a 55.8 kDa protein belonging to the plexin family. ; ; TEM7 is strongly expressed in endothelial cells from colorectal cancer and from primary cancers of the lung, liver, brain, breast and pancreas. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. ; ; Studies have indicated the existence of least four isoforms of TEM7 which include membrane bound, secreted and intracellular forms. ; ; TEM7 is not detectable in endothelial cells from normal tissues.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 500

• Applicable Applications for anti-TEM7 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 56kDa in rat liver cell lysates.; Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A peptide corresponding to a 16 amino acid sequence from near the center of human TEM7.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human TEM7 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of rat liver lysate probed with Rabbit anti Human TEM7 (MBS223982) at 0.5(A) and 1(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of mouse liver with Rabbit anti Human TEM7 (MBS223982) at 2.5ug/ml)

Related Product Information for anti-TEM7 antibody

MBS223982 recognises human Tumour endothelial marker 7 (TEM7), a 55.8 kDa protein belonging to the plexin family. TEM7 is strongly expressed in endothelial cells from colorectal cancer and from primary cancers of the lung, liver, brain, breast and pancreas. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Studies have indicated the existence of least four isoforms of TEM7 which include membrane bound, secreted and intracellular forms. TEM7 is not detectable in endothelial cells from normal tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 15011862
• NCBI GeneID: 57125
• NCBI Accession #: NP_065138.2
• NCBI GenBank Nucleotide #: NM_020405.4
• UniProt Accession #: Q8IUK5; Q5QCZ7; Q5QCZ8; Q5QCZ9; Q9HCT9; B2R7I8
• Molecular Weight: 47,895 Da
• NCBI Official Full Name: plexin domain-containing protein 1
• NCBI Official Synonym Full Names: plexin domain containing 1
• NCBI Official Symbol: PLXDC1
• NCBI Official Synonym Symbols: TEM3; TEM7
• NCBI Protein Information: plexin domain-containing protein 1; 2410003I07Rik; tumor endothelial marker 3; tumor endothelial marker 7
• UniProt Protein Name: Plexin domain-containing protein 1
• UniProt Gene Name: PLXDC1
• UniProt Synonym Gene Names: TEM3; TEM7
• UniProt Entry Name: PLDX1_HUMAN

Uniprot Description

PLXDC1: Plays a critical role in endothelial cell capillary morphogenesis. Belongs to the plexin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; tight junction; cell soma; cytoplasm; dendrite; extracellular region; integral to membrane; plasma membrane; intracellular; receptor complex

Molecular Function: protein binding; receptor activity

Biological Process: spinal cord development; angiogenesis