Rabbit TEM7 Polyclonal Antibody | anti-PLXDC1 antibody

TEM7 Antibody

Cat. Num. AAA150549

• Gene Names: PLXDC1; TEM3; TEM7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TEM7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TEM7; Polyclonal Antibody; TEM7 Antibody; TEM3; Plexin domain-containing protein 1; Tumor endothelial marker 3; plexin domain containing 1; anti-PLXDC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TEM7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 500

• Applicable Applications for anti-PLXDC1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TEM7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TEM7.
• Buffer: TEM7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TEM7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TEM7 in human liver tissue lysate with TEM7 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TEM7 in mouse liver tissue with TEM7 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TEM7 in mouse liver tissue with TEM7 antibody at 20 μg/mL.)

Related Product Information for anti-PLXDC1 antibody

TEM7 Antibody: Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.

NCBI and Uniprot Product Information

• NCBI GI #: 14017379
• NCBI GeneID: 57125
• UniProt Accession #: Q8IUK5; Q5QCZ7; Q5QCZ8; Q5QCZ9; Q9HCT9; B2R7I8
• Molecular Weight: 47,895 Da
• NCBI Official Full Name: tumor endothelial marker 7
• NCBI Official Synonym Full Names: plexin domain containing 1
• NCBI Official Symbol: PLXDC1
• NCBI Official Synonym Symbols: TEM3; TEM7
• NCBI Protein Information: plexin domain-containing protein 1; 2410003I07Rik; tumor endothelial marker 3; tumor endothelial marker 7
• UniProt Protein Name: Plexin domain-containing protein 1
• Protein Family: Plexin domain-containing protein
• UniProt Gene Name: PLXDC1
• UniProt Synonym Gene Names: TEM3; TEM7
• UniProt Entry Name: PLDX1_HUMAN

Uniprot Description

PLXDC1: Plays a critical role in endothelial cell capillary morphogenesis. Belongs to the plexin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; tight junction; cell soma; cytoplasm; dendrite; integral to membrane; plasma membrane; extracellular region; intracellular; receptor complex

Molecular Function: protein binding; receptor activity

Biological Process: spinal cord development; angiogenesis