Rabbit anti-Mouse, Rat IRGM Polyclonal Antibody | anti-IRGM antibody

RABBIT ANTI IRGM (INTERNAL REGION)

Cat. Num. AAA223612

• Gene Names: Irgm; Ifi1; Ifggd3
• Reactivity: Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: IRGM; Polyclonal Antibody; RABBIT ANTI IRGM (INTERNAL REGION); anti-IRGM antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 411

• Applicable Applications for anti-IRGM antibody: Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 32 kDa in human brain cell lysate.; Immunofluorescence: Maximum Dilution: 20ug/ml; Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to a 14 aa sequence from near the centre of human IRGM.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human IRGM was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human brain lysate probed with Rabbit anti Human IRGM (MBS223612) at 1(A) and 2(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of human brain with Rabbit anti Human IRGM (MBS223612))

Related Product Information for anti-IRGM antibody

Rabbit anti IRGM (Internal Region) antibody recognizes human Immunity-related GTPase family M protein (IRGM), also known as LRG47. IRGM is a putative GTPase required for clearance of bacterial and protozoan infections. It funtions in the innate immune response, most likely through regulation of autophagy. IRGM may also function in the regulation of proinflammatory cytokine production and in macrophage adhesion and motility.

NCBI and Uniprot Product Information

• NCBI GI #: 58865582
• NCBI GeneID: 303090
• NCBI Accession #: NP_001012007.1
• NCBI GenBank Nucleotide #: NM_001012007.1
• Molecular Weight: 46,338 Da
• NCBI Official Full Name: immunity-related GTPase family M protein
• NCBI Official Synonym Full Names: immunity-related GTPase family, M
• NCBI Official Symbol: Irgm
• NCBI Official Synonym Symbols: Ifi1; Ifggd3
• NCBI Protein Information: immunity-related GTPase family M protein; interferon inducible protein 1; interferon-gamma-inducible GTPase Ifggd3 protein
• UniProt Protein Name: Immunity-related GTPase family M protein
• Protein Family: Immunity-related GTPase family M protein
• UniProt Gene Name: Irgm
• UniProt Entry Name: IRGM_RAT

Uniprot Description

IRGM: Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19). A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Belongs to the interferon-inducible GTPase family.

Protein type: Endoplasmic reticulum; EC 3.6.5.-; Hydrolase

Cellular Component: Golgi membrane; phagocytic vesicle membrane; cell projection; lysosome; late endosome; phagocytic cup

Molecular Function: GTP binding; hydrolase activity

Biological Process: autophagy; innate immune response; defense response