Rabbit MD-2 Polyclonal Antibody | anti-MD-2 antibody

RABBIT ANTI MD-2

Cat. Num. AAA224037

• Gene Names: LY96; MD2; MD-2; ly-96; ESOP-1
• Applications: Immunohistochemistry, Western Blot
• Synonyms: MD-2; Polyclonal Antibody; RABBIT ANTI MD-2; anti-MD-2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 130

• Applicable Applications for anti-MD-2 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 26kDa in mouse spleen cell lysate; Immunohistology - Paraffin: Maximum Dilution: 2ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A peptide corresponding to amino acids near the middle of human MD-2.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to MD-2 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blotting analysis of mouse spleen lysate probed with Rabbit anti Human MD-2 (MBS223739))

Testing Data #2

(Immunohistochemical staining of rat spleen with Rabbit anti Human MD-2 (MBS223739))

Related Product Information for anti-MD-2 antibody

Rabbit anti MD-2 antibody recognizes MD-2, a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins which include Toll like receptors. TLR4 requires the binding of MD-2 to its extracellular region for best response to bacterial LPS. MD-2 also enhances TLR4-dependent activation of NF-kappa-beta and cooperates with TLR2 in the response to cell wall components from bacteria.

NCBI and Uniprot Product Information

• NCBI GI #: 307775409
• NCBI GeneID: 23643
• NCBI Accession #: NP_001182726.1
• NCBI GenBank Nucleotide #: NM_001195797.1
• Molecular Weight: 14,933 Da
• NCBI Official Full Name: lymphocyte antigen 96 isoform 2
• NCBI Official Synonym Full Names: lymphocyte antigen 96
• NCBI Official Symbol: LY96
• NCBI Official Synonym Symbols: MD2; MD-2; ly-96; ESOP-1
• NCBI Protein Information: lymphocyte antigen 96; myeloid differentiation protein-2; protein MD-2
• UniProt Protein Name: Lymphocyte antigen 96
• Protein Family: MD-2-related lipid-recognition protein
• UniProt Gene Name: LY96
• UniProt Synonym Gene Names: ESOP1; MD2; Ly-96
• UniProt Entry Name: LY96_HUMAN

NCBI Description

This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2010]

Uniprot Description

LY96: a secreted protein that binds to the extracellular domains of TLR2 and TLR4. Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS. Heterogeneous homopolymer formed from homodimers; disulfide-linked. Belongs to the lipopolysaccharide (LPS) receptor complex, a multi-protein complex containing at least CD14, LY96 and TLR4. Ligand binding induces interaction with TLR4 and oligomerization of the complex.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8q21.11

Cellular Component: extracellular space; plasma membrane; lipopolysaccharide receptor complex; endosome membrane

Molecular Function: protein binding; coreceptor activity; lipopolysaccharide receptor activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of lipopolysaccharide-mediated signaling pathway; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; cell surface receptor linked signal transduction; detection of lipopolysaccharide; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; cellular defense response; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway