Rabbit anti-Mouse, Rat AMBRA1 Polyclonal Antibody | anti-AMBRA1 antibody

RABBIT ANTI AMBRA1 (C-TERMINAL)

Cat. Num. AAA224119

• Gene Names: AMBRA1; DCAF3; WDR94
• Reactivity: Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Synonyms: AMBRA1; Polyclonal Antibody; RABBIT ANTI AMBRA1 (C-TERMINAL); anti-AMBRA1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1301

• Applicable Applications for anti-AMBRA1 antibody: Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunofluorescence: Maximum Dilution: 20ug/ml;; Immunohistology - Paraffin: Minimum Dilution: 5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to a 15 amino acid sequence from near the carboxy terminus of human AMBRA1.; Histology Positive Control Tissue
• Antiserum Preparation: Antiserum to human AMBRA1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from NIH-3T3 mouse embryonic fibroblast cells probed with Rabbit anti Human AMBRA1 (MBS224119) at 1ug/ml)

Testing Data #2

(Immunohistochemical staining of human brain with Rabbit anti Human AMBRA1 (MBS224119) at 5ug/ml)

Related Product Information for anti-AMBRA1 antibody

Rabbit anti AMBRA1 (C-Terminal) antibody recognizes human Activating molecule in beclin-1-regulated autophagy (AMBRA1), also known as WDR94. AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation. At least 5 isoforms of AMBRA have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 392306969
• NCBI GeneID: 55626
• NCBI Accession #: NP_001254711.1
• NCBI GenBank Nucleotide #: NM_001267782.1
• UniProt Accession #: Q9C0C7; Q86XD6; Q9H8Z0; Q9NXE7; A6XN33; D3DQP8; G3V193
• Molecular Weight: 84,428 Da
• NCBI Official Full Name: activating molecule in BECN1-regulated autophagy protein 1 isoform 1
• NCBI Official Synonym Full Names: autophagy/beclin-1 regulator 1
• NCBI Official Symbol: AMBRA1
• NCBI Official Synonym Symbols: DCAF3; WDR94
• NCBI Protein Information: activating molecule in BECN1-regulated autophagy protein 1; DDB1 and CUL4 associated factor 3; WD repeat domain 94; activating molecule in beclin-1-regulated autophagy
• UniProt Protein Name: Activating molecule in BECN1-regulated autophagy protein 1
• Protein Family: Activating molecule in BECN1-regulated autophagy protein
• UniProt Gene Name: AMBRA1
• UniProt Synonym Gene Names: KIAA1736
• UniProt Entry Name: AMRA1_HUMAN

Uniprot Description

AMBRA1: a WD40 protein that regulates autophagy and development of the nervous system. A highly conserved vertebrate protein whose expression is mostly confined to the neuroepithelium during early neurogenesis. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. Interacts with ATG6. Forms a complex with ATG6 and PIK3C3. Its functional inactivation in mouse is lethal in utero (starting from embryonic day 14.5), leading to severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, and excessive apoptosis. Hyperproliferation followed by a wave of caspase-dependent cell death was the earliest detectable abnormality in the developing neuroepithelium. Five isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Autophagy

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: mitochondrial outer membrane; mitochondrion; intracellular membrane-bound organelle; cytoplasm; autophagic vacuole; axoneme; phagocytic vesicle; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: neural tube development; negative regulation of cell proliferation; autophagy; positive regulation of phosphoinositide 3-kinase activity; negative regulation of neuron apoptosis; cell differentiation