Rabbit HRK Polyclonal Antibody | anti-HRK antibody

RABBIT ANTI HRK

Cat. Num. AAA223071

• Gene Names: HRK; DP5; HARAKIRI
• Applications: Western Blot
• Synonyms: HRK; Polyclonal Antibody; RABBIT ANTI HRK; anti-HRK antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation. ; Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 91

• Applicable Applications for anti-HRK antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 13kDa in mouse pancreas tissue lysate.; Western Blotting: Minimum Dilution: 2; Maximum Dilution: 5ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: 15 amino acid peptide from near the centre of human HRK (NP_003797).; Buffer Solution
• Antiserum Preparation: Antisera to HRK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse pancreas lysate probed with Rabbit anti Human Hrk (MBS221467) at 2.5(A) and 5(B) ug/ml)

Related Product Information for anti-HRK antibody

MBS221467 detects Harakiri (HRK), also known as DP5. HRK is a BH3 only protein of the Bcl-2 family, which promotes Bax dependent apoptosis. HRK regulates apoptosis through its interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL, and in conjunction to the mitochondrial pore forming protein p32. HRK is thought to have a specific role in initiating neuronal and haematopoietic cell apoptosis, in particular to growth factor deprivation. Aberrant methylation of HRK is frequently detected in primary colorectal cancers. Despite its predicted molecular weight, HRK often migrates at 12-15kD.

NCBI and Uniprot Product Information

• NCBI GI #: 4504493
• NCBI GeneID: 8739
• NCBI Accession #: NP_003797.1
• NCBI GenBank Nucleotide #: NM_003806.2
• UniProt Accession #: O00198
• Molecular Weight: 9,884 Da
• NCBI Official Full Name: activator of apoptosis harakiri
• NCBI Official Synonym Full Names: harakiri, BCL2 interacting protein
• NCBI Official Symbol: HRK
• NCBI Official Synonym Symbols: DP5; HARAKIRI
• NCBI Protein Information: activator of apoptosis harakiri; BCL2-interacting protein; BH3-interacting domain-containing protein 3; activator of apoptosis Hrk; death protein 5; harakiri, BCL2 interacting protein (contains only BH3 domain); neuronal death protein DP5
• UniProt Protein Name: Activator of apoptosis harakiri
• Protein Family: Activator of apoptosis harakiri
• UniProt Gene Name: HRK
• UniProt Synonym Gene Names: BID3
• UniProt Entry Name: HRK_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

HRK: Promotes apoptosis.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: positive regulation of protein homooligomerization; apoptosis; positive regulation of apoptosis