Western Blot (WB)
(Hrk-BH3 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Hrk antibody detected the Hrk protein (arrow).)
Rabbit anti-Human, mouse Hrk BH3 Domain Polyclonal Antibody | anti-HRK antibody
Hrk BH3 Domain Antibody
Gene Names
HRK; DP5; HARAKIRI
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Hrk BH3 Domain; Polyclonal Antibody; Hrk BH3 Domain Antibody; Activator of apoptosis harakiri; BH3-interacting domain-containing protein 3; Neuronal death protein DP5; HRK; BID3; anti-HRK antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Hrk BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-50 amino acids from human Hrk BH3 Domain.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
15-50
Sequence Length
91
Applicable Applications for anti-HRK antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Hrk-BH3 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Hrk antibody detected the Hrk protein (arrow).)
Western Blot (WB)
(Hrk-BH3 western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Hrk antibody detected the Hrk protein (arrow).)
Immunohistochemistry (IHC)
(Hrk BH3 Domain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hrk BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Hrk BH3 Domain Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Hrk BH3 Domain Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-HRK antibody
Activator of apoptosis Hrk regulates apoptosis through interaction with death-repressor proteins Bcl-2 and Bcl-X(L). The HRK protein lacks significant homology to other BCL2 family members except for an 8-amino acid region that was similar to the BCL2 homology domain-3 (BH3) motif of BIK. HRK interacts with BCL2 and BCLXL via the BH3 domain, but not with the death-promoting BCL2-related proteins BAX, BAK, or BCLXS. HRK localizes to membranes of intracellular organelles in a pattern similar to that previously reported for BCL2 and BCLXL.
Product Categories/Family for anti-HRK antibody
References
Wakabayashi, T., et al., Neurosci. Lett. 318(2):77-80 (2002).
Inohara, N., et al., EMBO J. 16(7):1686-1694 (1997).
Inohara, N., et al., EMBO J. 16(7):1686-1694 (1997).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9884
NCBI Official Full Name
activator of apoptosis harakiri
NCBI Official Synonym Full Names
harakiri, BCL2 interacting protein
NCBI Official Symbol
HRK
NCBI Official Synonym Symbols
DP5; HARAKIRI
NCBI Protein Information
activator of apoptosis harakiri
UniProt Protein Name
Activator of apoptosis harakiri
Protein Family
UniProt Gene Name
HRK
UniProt Synonym Gene Names
BID3
UniProt Entry Name
HRK_HUMAN
NCBI Description
This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
Uniprot Description
HRK: Promotes apoptosis.
Protein type: Apoptosis; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q24.22
Cellular Component: mitochondrion; integral to membrane
Molecular Function: protein binding
Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization
Research Articles on HRK
Similar Products
Product Notes
The HRK hrk (Catalog #AAA9212528) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 15-50. The Hrk BH3 Domain Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hrk BH3 Domain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the HRK hrk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hrk BH3 Domain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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