Rabbit TOSO Polyclonal Antibody | anti-TOSO antibody

RABBIT ANTI HUMAN TOSO (C-TERMINAL)

Cat. Num. AAA221472

• Gene Names: FAIM3; FCMR; TOSO
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Western Blot
• Synonyms: TOSO; Polyclonal Antibody; RABBIT ANTI HUMAN TOSO (C-TERMINAL); anti-TOSO antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the C-terminal (CT) region of human Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and haematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. ; ; Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kDa in SDS PAGE.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 278

• Applicable Applications for anti-TOSO antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 43kDa in human lung tissue lysate.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 13 amino acid peptide from near the carboxy terminus of human Toso.; Buffer Solution
• Antiserum Preparation: Antisera to human Toso were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human lung lysate probed with Rabbit anti Human TOSO (MBS221472))

Related Product Information for anti-TOSO antibody

MBS221472 detects an epitope within the C-terminal (CT) region of human Toso, also known as Fas Apoptotic Inhibitory Molecule 3 (FAIM3). Toso is expressed by lymphoid and haematopoietic cells and is up-regulated by T-cell activation. Toso expressing cells have increased resistance to Fas-, FADD-, and TNFalpha-induced apoptosis. Toso is membrane bound, and is thought to directly bind to FADD, disrupting DISC formation and thus inhibiting caspase-8 activation. Toso is a potential proto-oncogene, and could be a therapeutic target for use in the treatment of autoimmune diseases. Despite its predicted molecular weight, Toso often migrates at 60kDa in SDS PAGE.

NCBI and Uniprot Product Information

• NCBI GI #: 216547554
• NCBI GeneID: 9214
• NCBI Accession #: NP_001135945.1
• NCBI GenBank Nucleotide #: NM_001142473.1
• UniProt Accession #: O60667; A8K7J2; B7Z6Z0; D9MWM3
• Molecular Weight: 33,828 Da
• NCBI Official Full Name: fas apoptotic inhibitory molecule 3 isoform b
• NCBI Official Synonym Full Names: Fas apoptotic inhibitory molecule 3
• NCBI Official Symbol: FAIM3
• NCBI Official Synonym Symbols: FCMR; TOSO
• NCBI Protein Information: fas apoptotic inhibitory molecule 3; Fc mu receptor; IgM Fc receptor; immunoglobulin mu Fc receptor; regulator of Fas-induced apoptosis Toso
• UniProt Protein Name: Fas apoptotic inhibitory molecule 3
• UniProt Gene Name: FAIM3
• UniProt Synonym Gene Names: TOSO
• UniProt Entry Name: FAIM3_HUMAN

NCBI Description

Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al., 2009 [PubMed 19858324]; Shima et al., 2010 [PubMed 20042454]).[supplied by OMIM, Jul 2010]

Uniprot Description

FAIM3: May play a role in the immune system processes. Protects cells from FAS-, TNF alpha- and FADD-induced apoptosis without increasing expression of the inhibitors of apoptosis BCL2 and BCLXL. Seems to activate an inhibitory pathway that prevents CASP8 activation following FAS stimulation, rather than blocking apoptotic signals downstream. May inhibit FAS-induced apoptosis by preventing CASP8 processing through CFLAR up-regulation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: extracellular region; integral to membrane

Biological Process: immune system process; cellular defense response; negative regulation of apoptosis