Rabbit anti-Mouse, Rat PTK7 Polyclonal Antibody | anti-PTK7 antibody

RABBIT ANTI HUMAN PTK7 (N-TERMINAL)

Cat. Num. AAA222895

• Gene Names: PTK7; CCK4; CCK-4
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: PTK7; Polyclonal Antibody; RABBIT ANTI HUMAN PTK7 (N-TERMINAL); anti-PTK7 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human Protein tyrosine kinase-like 7 (PTK7), a member of the receptor tyrosine kinase family, otherwise known as Colon Carcinoma Kinase 4 (CCK4). PTK7 is highly expressed in the kidney, liver, lung, pancreas and melanocytes with weaker expression being detected in the thyroid gland, ovaries, brain, heart and skeletal muscle. ; ; PTK7 may play a role in embryonic development. It is thought to function as a cell adhesion molecule and as a novel regulator of the planar cell polarity (PCP) pathway in vertebrates. ; ; Studies also suggest that PTK7 may be involved in the tumour progression. A loss of PTK7 expression has been observed in melanoma cell lines, whereas increased levels of PTK7 have been detected in high-throughput acute myeloid leukaemia samples. In addition, PTK7 is not expressed in normal colon but has been detected in colon carcinomas and as such is a potential marker for tumour progression.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 1078

• Applicable Applications for anti-PTK7 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 86kDa in colon tissue lysates.; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; Preparation
• Immunogen: A 15 amino acid peptide located near the N-terminal region of human PTK7.; Buffer Solution
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of human (A) mouse (B) and Rat (C) colon lysates probed with Rabbit anti Human PTK7 (MBS222895) at 1ug/ml)

Testing Data #2

(Formalin fixed, paraffin embedded human colon stained with Rabbit anti Human PTK7 (MBS222895) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))

Related Product Information for anti-PTK7 antibody

MBS222895 recognises human Protein tyrosine kinase-like 7 (PTK7), a member of the receptor tyrosine kinase family, otherwise known as Colon Carcinoma Kinase 4 (CCK4). PTK7 is highly expressed in the kidney, liver, lung, pancreas and melanocytes with weaker expression being detected in the thyroid gland, ovaries, brain, heart and skeletal muscle. PTK7 may play a role in embryonic development. It is thought to function as a cell adhesion molecule and as a novel regulator of the planar cell polarity (PCP) pathway in vertebrates. Studies also suggest that PTK7 may be involved in the tumour progression. A loss of PTK7 expression has been observed in melanoma cell lines, whereas increased levels of PTK7 have been detected in high-throughput acute myeloid leukaemia samples. In addition, PTK7 is not expressed in normal colon but has been detected in colon carcinomas and as such is a potential marker for tumour progression.

NCBI and Uniprot Product Information

• NCBI GI #: 393715082
• NCBI GeneID: 5754
• NCBI Accession #: NP_001257327.1
• NCBI GenBank Nucleotide #: NM_001270398.1
• UniProt Accession #: Q13308; Q13417; Q5T650; Q6IQ54; Q8NFA5; Q8NFA6; Q8NFA7; Q8NFA8; A8K974; B7Z477; E9PFZ5
• Molecular Weight: 119,197 Da
• NCBI Official Full Name: inactive tyrosine-protein kinase 7 isoform e
• NCBI Official Synonym Full Names: protein tyrosine kinase 7 (inactive)
• NCBI Official Symbol: PTK7
• NCBI Official Synonym Symbols: CCK4; CCK-4
• NCBI Protein Information: inactive tyrosine-protein kinase 7; PTK7 protein tyrosine kinase 7; colon carcinoma kinase 4; pseudo tyrosine kinase receptor 7; tyrosine-protein kinase-like 7
• UniProt Protein Name: Inactive tyrosine-protein kinase 7
• Protein Family: Inactive tyrosine-protein kinase
• UniProt Gene Name: PTK7
• UniProt Synonym Gene Names: CCK4; CCK-4
• UniProt Entry Name: PTK7_HUMAN

NCBI Description

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Membrane protein, integral; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; CCK4 family

Chromosomal Location of Human Ortholog: 6p21.1-p12.2

Cellular Component: focal adhesion; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; protein-tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; cell adhesion; signal transduction