Rabbit PTK7 Polyclonal Antibody | anti-PTK7 antibody

PTK7 Antibody

Cat. Num. AAA151466

• Gene Names: PTK7; CCK4; CCK-4
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PTK7 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PTK7; Polyclonal Antibody; PTK7 Antibody; CCK4; CCK-4; Inactive tyrosine-protein kinase 7; Colon carcinoma kinase 4; PTK7 protein tyrosine kinase 7; anti-PTK7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PTK7 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1070

• Applicable Applications for anti-PTK7 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PTK7 antibody can be used for detection of PTK7 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PTK7 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human PTK7.
• Buffer: PTK7 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PTK7 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PTK7 in (A) human colon, (B) mouse kidney and (C) rat liver tissue lysate with PTK7 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PTK7 in human colon tissue with PTK7 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PTK7 in Human Colon cells with PTK7 antibody at 20 μg/mL.)

Related Product Information for anti-PTK7 antibody

PTK7 Antibody: Protein-tyrosine kinases (PTKs) play important roles in regulating cell proliferation and differentiation during development. One member of the PTK family, PTK7, has been suggested to regulate the planar cell polarity (PCP) pathway in vertebrates and may play a role in neural convergent extension and neural tube closure. PTK7 has also been implicated in the development of cancer. Loss of PTK7 expression was seen in several melanoma cell lines and biopsies. Conversely, high-throughput analysis of acute myeloid leukemia samples showed an increased level of PTK7 expression compared to normal bone marrow and purified CD34+ cells. Multiple isoforms of PTK7 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 47938093
• NCBI GeneID: 5754
• UniProt Accession #: Q13308; Q13417; Q5T650; Q6IQ54; Q8NFA5; Q8NFA6; Q8NFA7; Q8NFA8; A8K974; B7Z477; E9PFZ5
• Molecular Weight: 119,197 Da
• NCBI Official Full Name: PTK7 protein tyrosine kinase 7
• NCBI Official Synonym Full Names: protein tyrosine kinase 7 (inactive)
• NCBI Official Symbol: PTK7
• NCBI Official Synonym Symbols: CCK4; CCK-4
• NCBI Protein Information: inactive tyrosine-protein kinase 7; colon carcinoma kinase 4; PTK7 protein tyrosine kinase 7; tyrosine-protein kinase-like 7; pseudo tyrosine kinase receptor 7
• UniProt Protein Name: Inactive tyrosine-protein kinase 7
• Protein Family: Inactive tyrosine-protein kinase
• UniProt Gene Name: PTK7
• UniProt Synonym Gene Names: CCK4; CCK-4
• UniProt Entry Name: PTK7_HUMAN

NCBI Description

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Kinase, protein; TK group; CCK4 family

Chromosomal Location of Human Ortholog: 6p21.1-p12.2

Cellular Component: focal adhesion; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; protein-tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; signal transduction; cell adhesion