Human PTK7 protein tyrosine kinase 7 ELISA Kit | PTK7 elisa kit

Human Inactive tyrosine-protein kinase 7, PTK7 ELISA Kit

Cat. Num. AAA937713

• Gene Names: PTK7; CCK4; CCK-4
• Reactivity: Human
• Synonyms: PTK7 protein tyrosine kinase 7; Human Inactive tyrosine-protein kinase 7; PTK7 ELISA Kit; Human Inactive tyrosine-protein kinase 7 (PTK7) ELISA kit; CCK4; colon carcinoma kinase-4; PTK7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PTK7. No significant cross-reactivity or interference between human PTK7 and analogues was observed.
• Sequence Length: 1078

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: < 3.125 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTK7 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTK7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 393715082
• NCBI GeneID: 5754
• NCBI Accession #: NP_001257327.1
• NCBI GenBank Nucleotide #: NM_001270398.1
• UniProt Accession #: Q13308; Q13417; Q5T650; Q6IQ54; Q8NFA5; Q8NFA6; Q8NFA7; Q8NFA8; A8K974; B7Z477; E9PFZ5
• Molecular Weight: 118,392 Da
• NCBI Official Full Name: inactive tyrosine-protein kinase 7 isoform e
• NCBI Official Synonym Full Names: protein tyrosine kinase 7
• NCBI Official Symbol: PTK7
• NCBI Official Synonym Symbols: CCK4; CCK-4
• NCBI Protein Information: inactive tyrosine-protein kinase 7; colon carcinoma kinase 4; PTK7 protein tyrosine kinase 7; tyrosine-protein kinase-like 7; pseudo tyrosine kinase receptor 7
• UniProt Protein Name: Inactive tyrosine-protein kinase 7
• Protein Family: Inactive tyrosine-protein kinase
• UniProt Gene Name: PTK7
• UniProt Synonym Gene Names: CCK4; CCK-4
• UniProt Entry Name: PTK7_HUMAN

NCBI Description

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TK; Membrane protein, integral; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; CCK4 family

Chromosomal Location of Human Ortholog: 6p21.1-p12.2

Cellular Component: focal adhesion; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; protein-tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; signal transduction; cell adhesion