Rabbit CCK4 / PTK7 Polyclonal Antibody | anti-CCK4 antibody

Rabbit Polyclonal to Human CCK4 / PTK7

Cat. Num. AAA243178

• Gene Names: PTK7; CCK4; CCK-4
• Reactivity: Bovine, Chimpanzee, Chicken, Dog, Horse, Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CCK4 / PTK7; Polyclonal Antibody; Rabbit Polyclonal to Human CCK4 / PTK7; Anti-CCK4 / PTK7 Antibody IHC-plus; PTK7; CCK-4; CCK4; Colon carcinoma kinase 4; Tyrosine-protein kinase-like 7; Protein-tyrosine kinase 7; PTK7 protein tyrosine kinase 7; Human CCK4; anti-CCK4 antibody

• Host: Rabbit
• Reactivity: Bovine, Chimpanzee, Chicken, Dog, Horse, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: A portion of amino acid 900-950 of human PTK7 protein
• Form/Format: Crude rabbit sera with 50% glycerol and 0.025% sodium azide.
• Sequence Length: 1070

• Applicable Applications for anti-CCK4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (1:200), WB (1:5000)
• Usage Not Recommended: Immunohistchemistry (IHC-P)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human PTK7.
• Immunogen Type: Synthetic peptide
• Immunogen: CCK4 / PTK7 antibody was raised against synthetic peptide from human PTK7.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-PTK7 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Western Blot (WB)

(Western blot of PTK7 in HCT116 cell lysate. Lane 1 shows pre-immune sera. Lane 2 shows antibody tested at 1:5000 dilution.)

Related Product Information for anti-CCK4 antibody

PTK7, a CCK4 membrane spanning tyrosine protein kinase, belongs to a subgroup of kinases that lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. PTK7 may function as a cell adhesion molecule. This gene was isolated from carcinomic colon tissue and has been suggested to affect colon carcinoma physiology. Expression of PTK7 has been shown to be reduced in melanoma samples.

Product Categories/Family for anti-CCK4 antibody

Family: Protein Kinase; Subfamily: CCK4

NCBI and Uniprot Product Information

• NCBI GI #: 15826840
• NCBI GeneID: 5754
• NCBI Accession #: NP_002812.2
• NCBI GenBank Nucleotide #: NM_002821.4
• UniProt Accession #: Q13308; Q13417; Q5T650; Q6IQ54; Q8NFA5; Q8NFA6; Q8NFA7; Q8NFA8; A8K974; B7Z477; E9PFZ5
• Molecular Weight: 119,197 Da
• NCBI Official Full Name: inactive tyrosine-protein kinase 7 isoform a
• NCBI Official Synonym Full Names: protein tyrosine kinase 7 (inactive)
• NCBI Official Symbol: PTK7
• NCBI Official Synonym Symbols: CCK4; CCK-4
• NCBI Protein Information: inactive tyrosine-protein kinase 7; colon carcinoma kinase 4; PTK7 protein tyrosine kinase 7; tyrosine-protein kinase-like 7; pseudo tyrosine kinase receptor 7
• UniProt Protein Name: Inactive tyrosine-protein kinase 7
• UniProt Gene Name: PTK7
• UniProt Synonym Gene Names: CCK4; CCK-4
• UniProt Entry Name: PTK7_HUMAN

NCBI Description

This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

CCK4: Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (receptor); Membrane protein, integral; EC 2.7.10.1; TK group; CCK4 family

Chromosomal Location of Human Ortholog: 6p21.1-p12.2

Cellular Component: focal adhesion; integral to plasma membrane; intercellular junction

Molecular Function: protein binding; protein-tyrosine kinase activity; ATP binding

Biological Process: cell migration; peptidyl-tyrosine phosphorylation; wound healing; convergent extension; actin cytoskeleton reorganization; neural tube closure; establishment of epithelial cell polarity; establishment of planar polarity; Wnt receptor signaling pathway through beta-catenin; signal transduction; cell adhesion