Rabbit ERK/MAPK Polyclonal Antibody | anti-ERK/MAPK antibody

RABBIT ANTI ERK/MAPK (pThr202/pTyr204)

Cat. Num. AAA221791

• Gene Names: Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human, Monkey (Expected from Sequence), Mouse (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)
• Applications: Western Blot
• Synonyms: ERK/MAPK; Polyclonal Antibody; RABBIT ANTI ERK/MAPK (pThr202/pTyr204); anti-ERK/MAPK antibody

• Host: Rabbit
• Reactivity: Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human, Monkey (Expected from Sequence), Mouse (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for the ~42-44 kDa extracellular-signal regulated kinase/mitogen activated protein kinase (ERK/MAPK), when phosphorylated at threonine 202 and tyrosine 204. ERK/MAPK is an important integration point of many signalling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.
• Form/Format: Purified; Purified IgG - liquid
• Sequence Length: 380

• Applicable Applications for anti-ERK/MAPK antibody: Western Blot (WB)*
• Application Notes: Western Blot: This item recognises a band of approximately 43kD in human T47D lysates.; Western Blotting: Maximum Dilution: 1/1000; Application Note: For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
• Perservative Stabilisers: 0.09% Sodium Azide; 0.01% Bovine Serum Albumin; 50% Glycerol
• Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within rat ERK/MAPK which includes phosphorylated Thr202 and Tyr204.; Buffer Solution
• Antiserum Preparation: Antisera to phosphorylated rat ERK/MAPK were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Rat
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Related Product Information for anti-ERK/MAPK antibody

MBS221791 is specific for the ~42-44 kDa extracellular-signal regulated kinase/mitogen activated protein kinase (ERK/MAPK), when phosphorylated at threonine 202 and tyrosine 204. ERK/MAPK is an important integration point of many signalling pathways, for a range of cellular processes including proliferation, differentiation, transcription regulation and development. Upon phosphorylation, activated ERK/MAPK translocates to the nucleus, where it phosphorylates nuclear targets.

NCBI and Uniprot Product Information

• NCBI GI #: 8393331
• NCBI GeneID: 50689
• NCBI Accession #: NP_059043.1
• NCBI GenBank Nucleotide #: NM_017347.2
• Molecular Weight: 45,770 Da
• NCBI Official Full Name: mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen activated protein kinase 3
• NCBI Official Symbol: Mapk3
• NCBI Official Synonym Symbols: p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• NCBI Protein Information: mitogen-activated protein kinase 3; MAP kinase 1; MAP kinase 3; MAP kinase isoform p44; MAPK 1; MAPK 3; extracellular signal-regulated kinase 1; insulin-stimulated MAP2 kinase; microtubule-associated protein 2 kinase; mitogen-activated 3; mitogen-activated protein kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: Mapk3
• UniProt Synonym Gene Names: Erk1; Prkm3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_RAT

NCBI Description

kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Cellular Component: Golgi apparatus; protein complex; focal adhesion; mitochondrion; early endosome; nuclear envelope; caveola; pseudopodium; cytosol; microtubule cytoskeleton; nucleoplasm; cytoskeleton; cytoplasm; late endosome; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; phosphotyrosine binding; ATP binding; protein kinase activity; phosphatase binding

Biological Process: positive regulation of histone phosphorylation; DNA damage induced protein phosphorylation; positive regulation of translation; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; sensory perception of pain; protein amino acid phosphorylation; BMP signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; lipopolysaccharide-mediated signaling pathway; arachidonic acid metabolic process; protein complex assembly; transcription, DNA-dependent; MAPKKK cascade; regulation of stress-activated MAPK cascade; peptidyl-serine phosphorylation; nuclear translocation of MAPK; organ morphogenesis; cartilage development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; phosphorylation; regulation of cytoskeleton organization and biogenesis