Rabbit Erk1/2 Polyclonal Antibody | anti-Mapk3 antibody

Erk1/2 Antibody

Cat. Num. AAA806473

• Gene Names: Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• Reactivity: Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Synonyms: Erk1/2; Polyclonal Antibody; Erk1/2 Antibody; ERK1; ERK2; ERT1; ERT2; MAP kinase1; MAP kinase2; MAPK1; MAPK2; MAPK3; p38; p40; p41; p41mapk; p42 MAPK; p44 ERK1; p44 MAPK; PRKM1 PRKM2; PRKM3; anti-Mapk3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Cow, Sheep, Chicken, Drosophila, Xenopus
• Clonality: Polyclonal
• Form/Format: Rabbit Antiserum
• Sequence Length: 380

• Applicable Applications for anti-Mapk3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: 1:5000 (ECL) (WB), 1:50 (IHC), 10ug/mL (Flow cytometry)
• Certificate of Analysis: A 1:5000 dilution of SPC-120 was sufficient for detection of ERK1/2 in 20ug of HeLa cell lysate by ECL immunoblot analysis.
• Conjugate: Unconjugated
• Storage Buffer: Rabbit antiserum
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western blot analysis of Human Cell line lysates showing detection of ERK1 protein using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:25000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cytoplasm. Nucleus.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-ERK1 Polyclonal Antibody (SPC-120) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)

Related Product Information for anti-Mapk3 antibody

Background Info: Detects ~44kda and ~42kDa bands corresponding to the molecular weights of Erk1 and Erk2.

Scientific Background: The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase (1). They are both located in the cytosol and mitochondria (2). While the role of cytosol ERK1/2 is well studied and involved in multiple cellular functions (2), the role of mitochondrial ERK1/2 remains poorly understood. Both ERK 1 and 2 are activated by MEK1 or MEK2, by dual phosphorylation of a threonine and tyrosine residue in the activation loop (TEY motif) (1, 3). Either phosphorylation alone can induce an electrophoretic mobility shift, but both are required for activation of the kinase. This dual phosphorylation is efficiently detected by phosphorylation state-specific antibody directed to the pTEpY motif. Once activated, MAP kinases phosphorylate a broad spectrum of substrates, including cytoskeletal proteins, translation regulators, transcription factors, and the Rsk family of protein kinases (4). ERK1/2 activation is generally thought to confer a survival advantage to cells (5); however there is increasing evidence that suggests that the activation of ERK1/2 also contributes to cell death under certain conditions (5). ERK1/2 also is activated in neuronal and renal epithelial cells upon exposure to oxidative stress and toxicants or deprivation of growth factors, and inhibition of the ERK pathway blocks apoptosis (5).

Product Categories/Family for anti-Mapk3 antibody

Cell Signaling

References

1. Boulton TG. et al. (1991) Biochemistry. 30(1):278-86. 2. Yoon S., and Seger R. (2006) Growth Factors 24:21-44. 3. Wolf G. (2005) Antioxid Redox Signal 7:1337-1345. 4. Chuerland D., Marmor G., Shainskaya A. and Seger R. (2008) J Biol Chem. Epub: http://www.jbc.org/cgi/doi/10.1074/jbc.M709030200 5. Zhuang S., and Schnellmann R.G. (2006) J Pharmacol Exp Ther 319:991-997.

NCBI and Uniprot Product Information

• NCBI GI #: 8393331
• NCBI GeneID: 50689
• NCBI Accession #: NP_059043.1
• NCBI GenBank Nucleotide #: NM_017347.2
• UniProt Accession #: P21708; Q4PIY8; Q62686; Q9JJ13
• Molecular Weight: 45,770 Da
• NCBI Official Full Name: mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen activated protein kinase 3
• NCBI Official Symbol: Mapk3
• NCBI Official Synonym Symbols: p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
• NCBI Protein Information: mitogen-activated protein kinase 3; MAPK 1; MAPK 3; MAP kinase 1; MAP kinase 3; mitogen-activated 3; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; mitogen-activated protein kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: Mapk3
• UniProt Synonym Gene Names: Erk1; Prkm3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_RAT

NCBI Description

kinase involved in intracellular signalling; component of Mapk signalling pathway [RGD, Feb 2006]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily

Cellular Component: Golgi apparatus; focal adhesion; protein complex; mitochondrion; early endosome; nuclear envelope; pseudopodium; caveola; cytosol; microtubule cytoskeleton; nucleoplasm; cytoskeleton; cytoplasm; late endosome; nucleus

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; phosphotyrosine binding; ATP binding; protein kinase activity; phosphatase binding

Biological Process: positive regulation of histone phosphorylation; DNA damage induced protein phosphorylation; positive regulation of translation; apoptosis; response to toxin; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; sensory perception of pain; protein amino acid phosphorylation; BMP signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; lipopolysaccharide-mediated signaling pathway; arachidonic acid metabolic process; protein complex assembly; transcription, DNA-dependent; MAPKKK cascade; regulation of stress-activated MAPK cascade; organ morphogenesis; nuclear translocation of MAPK; peptidyl-serine phosphorylation; cartilage development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of histone acetylation; positive regulation of protein amino acid phosphorylation; phosphorylation; response to DNA damage stimulus; regulation of cytoskeleton organization and biogenesis