Rabbit PPAR ALPHA Polyclonal Antibody | anti-PPAR ALPHA antibody

RABBIT ANTI HUMAN PPAR ALPHA (N-TERMINAL)

Cat. Num. AAA221542

• Gene Names: PPARA; PPAR; NR1C1; hPPAR; PPARalpha
• Applications: ELISA, Western Blot
• Synonyms: PPAR ALPHA; Polyclonal Antibody; RABBIT ANTI HUMAN PPAR ALPHA (N-TERMINAL); anti-PPAR ALPHA antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA), a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle. ; ; PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux. ; ; PPARA is the main target of fibrate drugs; hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-PPAR ALPHA antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 52kDa in 3T3 whole cell lysate.; ELISA: Minimum Dilution: 1/8000; Maximum Dilution: 1/32000; Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide corresponding to amino acids 1-18 of human PPAR alpha.; Buffer Solution
• Antiserum Preparation: Antisera to human PPAR alpha were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-PPAR ALPHA antibody

MBS221542 detects an epitope within the N-terminal region of human peroxisome proliferator-activated receptor, alpha (PPARA), a member of the nuclear hormone receptor subfamily. Upon activation, PPARA forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. In particular, PPARA controls genes involved in the peroxisomal and mitochondrial beta oxidation pathways, fatty acid uptake, and triglyceride catabolism in the liver and skeletal muscle. PPARA also has anti-inflammatory and anti-proliferative effects and prevents the proatherogenic effects of cholesterol accumulation in macrophages by stimulating cholesterol efflux. PPARA is the main target of fibrate drugs; hypolipidemic agents used in the treatment of dyslipidemia and the prevention of cardiovascular disease. They could also be used for the treatment of type II diabetes.

NCBI and Uniprot Product Information

• NCBI GI #: 50348666
• NCBI GeneID: 5465
• NCBI Accession #: NP_001001928.1
• NCBI GenBank Nucleotide #: NM_001001928.2
• UniProt Accession #: Q07869; Q16241; Q6I9S0; Q92486; Q92689; Q9Y3N1; B0G0X3
• Molecular Weight: 18,942 Da
• NCBI Official Full Name: peroxisome proliferator-activated receptor alpha
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor alpha
• NCBI Official Symbol: PPARA
• NCBI Official Synonym Symbols: PPAR; NR1C1; hPPAR; PPARalpha
• NCBI Protein Information: peroxisome proliferator-activated receptor alpha; PPAR-alpha; nuclear receptor subfamily 1 group C member 1; peroxisome proliferative activated receptor, alpha; peroxisome proliferator-activated nuclear receptor alpha variant 3
• UniProt Protein Name: Peroxisome proliferator-activated receptor alpha
• UniProt Gene Name: PPARA
• UniProt Synonym Gene Names: NR1C1; PPAR; PPAR-alpha
• UniProt Entry Name: PPARA_HUMAN

NCBI Description

Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PPAR-alpha: Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein domain specific binding; ligand-dependent nuclear receptor activity; protein binding; NFAT protein binding; DNA binding; zinc ion binding; ubiquitin conjugating enzyme binding; sequence-specific DNA binding; protein complex binding; steroid hormone receptor activity; drug binding; lipid binding; transcription factor activity; phosphatase binding

Biological Process: circadian rhythm; epidermis development; wound healing; heart development; positive regulation of transcription, DNA-dependent; behavioral response to nicotine; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; fatty acid metabolic process; negative regulation of appetite; response to insulin stimulus; positive regulation of gluconeogenesis; negative regulation of blood pressure; circadian regulation of gene expression; negative regulation of glycolysis; negative regulation of protein binding; transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; lipoprotein metabolic process; regulation of circadian rhythm; positive regulation of fatty acid beta-oxidation; positive regulation of fatty acid oxidation; response to hypoxia; steroid hormone mediated signaling; gene expression; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; fatty acid transport