Rabbit IL-10 Polyclonal Antibody | anti-Il10 antibody

RABBIT ANTI MOUSE INTERLEUKIN-10

Cat. Num. AAA221262

• Gene Names: Il10; CSIF; Il-10
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: IL-10; Polyclonal Antibody; RABBIT ANTI MOUSE INTERLEUKIN-10; anti-Il10 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml after reconstitution. (varies by lot)
• Sequence Length: 178

• Applicable Applications for anti-Il10 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml;; Functional Assays: Minimum Dilution: 0.02; Maximum Dilution: 0.04ug/ml;; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
• Immunogen: Recombinant mouse IL-10; Buffer Solution
• Antiserum Preparation: Antisera to mouse IL-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-Il10 antibody

Rabbit anti Mouse Interleukin-10 antibody recognizes mouse Interleukin-10 (IL-10), an immunosuppressive cytokine originally isolated from T helper type 2 cells (Th2), which is also secreted by B lymphocytes, macrophages, monocytes and keratinocytes. IL-10 inhibits the synthesis of several cytokines including Tumour Necrosis Factor, Interferon-gamma and Interleukin-2 and -3, and has a wide range of biological properties, including inhibition of the macrophage and Th cell-mediated cytokine synthesis suppression of the delayed hypersensitivity response. IL-10 may initially be involved in the polarization of Th2 responses.

NCBI and Uniprot Product Information

• NCBI GI #: 6754318
• NCBI GeneID: 16153
• NCBI Accession #: NP_034678.1
• NCBI GenBank Nucleotide #: NM_010548.2
• UniProt Accession #: P18893; Q0VBJ1
• Molecular Weight: 20,641 Da
• NCBI Official Full Name: interleukin-10
• NCBI Official Synonym Full Names: interleukin 10
• NCBI Official Symbol: Il10
• NCBI Official Synonym Symbols: CSIF; Il-10
• NCBI Protein Information: interleukin-10; cytokine synthesis inhibitory factor
• UniProt Protein Name: Interleukin-10
• Protein Family: Interleukin
• UniProt Gene Name: Il10
• UniProt Synonym Gene Names: Il-10; IL-10; CSIF
• UniProt Entry Name: IL10_MOUSE

Uniprot Description

IL10: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. Belongs to the IL-10 family.

Protein type: Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: cytokine activity; interleukin-10 receptor binding

Biological Process: negative regulation of chronic inflammatory response to antigenic stimulus; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of JAK-STAT cascade; negative regulation of B cell proliferation; negative regulation of membrane protein ectodomain proteolysis; negative regulation of interferon-gamma production; response to molecule of bacterial origin; positive regulation of MHC class II biosynthetic process; negative regulation of interleukin-6 production; negative regulation of tumor necrosis factor biosynthetic process; inflammatory response; negative regulation of interleukin-12 production; positive regulation of B cell apoptosis; negative regulation of nitric oxide biosynthetic process; negative regulation of myeloid dendritic cell activation; negative regulation of tumor necrosis factor production; negative regulation of cytokine secretion during immune response; regulation of gene expression; negative regulation of inflammatory response; negative regulation of cytokine production; defense response to bacterium; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; immune response; receptor biosynthetic process; regulation of sensory perception of pain; positive regulation of cytokine secretion; negative regulation of apoptosis