Sheep INDOLEAMINE 2,3-DIOXYGENASE Polyclonal Antibody | anti-IDO1 antibody

SHEEP ANTI HUMAN INDOLEAMINE 2,3-DIOXYGENASE

Cat. Num. AAA220490

• Gene Names: IDO1; IDO; INDO; IDO-1
• Reactivity: Marmoset, Rhesus Monkey
• Applications: Immunohistochemistry, Western Blot
• Synonyms: INDOLEAMINE 2; 3-DIOXYGENASE; Polyclonal Antibody; SHEEP ANTI HUMAN INDOLEAMINE 2; anti-IDO1 antibody

• Host: Sheep
• Reactivity: Marmoset, Rhesus Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
• Form/Format: Ig Fraction; Ig Fraction - liquid
• Concentration: Total protein concentration 27.1 mg/ml (varies by lot)
• Sequence Length: 403

• Applicable Applications for anti-IDO1 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.09% Sodium Azide
• Immunogen: Recombinant human indoleamine 2,3-dioxygenase.; Buffer Solution
• Antiserum Preparation: Antisera to human indoleamine dioxygenase (IDO) were raised by repeated immunisation of sheep with highly purified antigen. Ig fraction prepared by ammonium sulphate precipitation.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-IDO1 antibody

MBS220490 recognises human indoleamine 2,3-dioxygenase (IDO), an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. Reports suggest that IDO is involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.

NCBI and Uniprot Product Information

• NCBI GI #: 4504577
• NCBI GeneID: 3620
• NCBI Accession #: NP_002155.1
• NCBI GenBank Nucleotide #: NM_002164.5
• UniProt Accession #: P14902; Q540B4
• Molecular Weight: 45,326 Da
• NCBI Official Full Name: indoleamine 2,3-dioxygenase 1
• NCBI Official Synonym Full Names: indoleamine 2,3-dioxygenase 1
• NCBI Official Symbol: IDO1
• NCBI Official Synonym Symbols: IDO; INDO; IDO-1
• NCBI Protein Information: indoleamine 2,3-dioxygenase 1; indolamine 2,3 dioxygenase; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
• UniProt Protein Name: Indoleamine 2,3-dioxygenase 1
• Protein Family: Indoleamine 2,3-dioxygenase
• UniProt Gene Name: IDO1
• UniProt Synonym Gene Names: IDO; INDO; IDO-1
• UniProt Entry Name: I23O1_HUMAN

NCBI Description

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: EC 1.13.11.52; Oxidoreductase; Amino Acid Metabolism - tryptophan; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8p12-p11

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytosol

Molecular Function: electron carrier activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; tryptophan 2,3-dioxygenase activity

Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction