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Immunohistochemistry (IHC) (Immunohistochemistry Analysis: (Figures 1 & 2): Representative positive and background staining patterns on formalin-fixed, paraffin-embedded placental syncytiotrophoblast cells. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Antibody diluted to 1:1-500. Immunoreactivity is seen as strong staining of placental syncytiotrophoblast cells. Figure 1 (Low Magnification). Figure 2 (High Magnification).)

Mouse anti-Human Indoleamine 2,3-Dioxygenase Monoclonal Antibody | anti-Ido1 antibody

Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase, INDO, Indole 2,3 dioxygenase, HGNC:6059)

Gene Names
Ido1; Ido; Indo
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Synonyms
Indoleamine 2; 3-Dioxygenase; Monoclonal Antibody; 3-Dioxygenase (IDO; Indoleamine-pyrrole 2; 3-dioxygenase; INDO; Indole 2; 3 dioxygenase; HGNC:6059); Anti -Indoleamine 2; anti-Ido1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
9i171
Specificity
Recognizes human IDO.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-Ido1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.1ug/ml
Immunohistochemistry: 1:1-500
Immunogen
GST-tagged recombinant protein corresponding to residues surrounding IDO.
Positive Control
HeLa cell lysate treated with interferon gamma
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry Analysis: (Figures 1 & 2): Representative positive and background staining patterns on formalin-fixed, paraffin-embedded placental syncytiotrophoblast cells. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Antibody diluted to 1:1-500. Immunoreactivity is seen as strong staining of placental syncytiotrophoblast cells. Figure 1 (Low Magnification). Figure 2 (High Magnification).)

Immunohistochemistry (IHC) (Immunohistochemistry Analysis: (Figures 1 & 2): Representative positive and background staining patterns on formalin-fixed, paraffin-embedded placental syncytiotrophoblast cells. Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques. Antibody diluted to 1:1-500. Immunoreactivity is seen as strong staining of placental syncytiotrophoblast cells. Figure 1 (Low Magnification). Figure 2 (High Magnification).)

Western Blot (WB)

(Western Blot Analysis: Representative lot data. HeLa + Interferon gamma cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS608213. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IDO (45kD).)

Western Blot (WB) (Western Blot Analysis: Representative lot data. HeLa + Interferon gamma cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS608213. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IDO (45kD).)
Related Product Information for anti-Ido1 antibody
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumors and blocking the IDO pathway may be a potential target for immunotherapy.
Product Categories/Family for anti-Ido1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
45,641 Da
NCBI Official Full Name
indoleamine 2,3-dioxygenase
NCBI Official Synonym Full Names
indoleamine 2,3-dioxygenase 1
NCBI Official Symbol
Ido1
NCBI Official Synonym Symbols
Ido; Indo
NCBI Protein Information
indoleamine 2,3-dioxygenase 1; IDO-1; OTTMUSP00000022551; OTTMUSP00000022552; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase
UniProt Protein Name
Indoleamine 2,3-dioxygenase 1
UniProt Gene Name
Ido1
UniProt Synonym Gene Names
Ido; Indo
UniProt Entry Name
I23O1_MOUSE

Uniprot Description

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.

Protein type: Cell cycle regulation; Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Oxidoreductase

Cellular Component: stereocilium bundle; smooth muscle contractile fiber; cytoplasm; cytosol

Molecular Function: amino acid binding; protein binding; dioxygenase activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity

Biological Process: positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; tryptophan catabolic process to kynurenine; positive regulation of apoptosis; positive regulation of interleukin-12 production; negative regulation of activated T cell proliferation; response to lipopolysaccharide; defense response; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction

Research Articles on Ido1

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Product Notes

The Ido1 ido1 (Catalog #AAA608213) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Indoleamine 2,3-Dioxygenase (IDO, Indoleamine-pyrrole 2,3-dioxygenase, INDO, Indole 2,3 dioxygenase, HGNC:6059) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Indoleamine 2,3-Dioxygenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.1ug/ml Immunohistochemistry: 1:1-500. Researchers should empirically determine the suitability of the Ido1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indoleamine 2,3-Dioxygenase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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