Goat VEGF Polyclonal Antibody | anti-VEGF antibody

GOAT ANTI HUMAN VEGF:Biotin

Cat. Num. AAA220220

• Gene Names: VEGFA; VPF; VEGF; MVCD1
• Applications: ELISA, Western Blot
• Synonyms: VEGF; Polyclonal Antibody; GOAT ANTI HUMAN VEGF:Biotin; anti-VEGF antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Biotin; Purified IgG conjugated to Biotin - lyophilised
• Concentration: IgG concentration 0.1 mg/ml after reconstitution. (varies by lot)
• Sequence Length: 412

• Applicable Applications for anti-VEGF antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: This biotinylated human VEGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human VEGF antibody as the capture reagent and recombinant human VEGF as the standard.; Western Blot: This antibody may be used in Western Blotting under either reducing or non-reducing conditions with recombinant human VEGF as the positive control.; ELISA: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage, the addition of 0.09% sodium azide is recommended.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human VEGF were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-VEGF antibody

Goat anti Human VEGF antibody specifically recognizes human VEGF (vascular endothelial growth factor), a heparin-binding cytokine and member of the PDGF/VEGF growth factor family, which exists as several splice variants of the VEGF gene. VEGF, originally named vascular permeability factor due to its ability to act as vasodilator and increase microvascular permeability, is a potent stimulator of angiogenesis of both normal and cancerous cells, and acts as a regulator of vasculogenesis. The functional forms of VEGF exist as disulphide-linked homodimers and are classified according to the number of amino acid residues within a constitutive polypeptide chain e.g VMBS23026165. All VEGF family members act through binding to the tyrosine kinase receptors VEGFR-1 (Flt-1) and VEGFR-2 (KDR/CD309), whilst the closely-related VEGF-C and VEGF-D, can also bind to VEGFR-3 (Flt-4).

NCBI and Uniprot Product Information

• NCBI GI #: 76781480
• NCBI GeneID: 7422
• NCBI Accession #: NP_001020537.2
• NCBI GenBank Nucleotide #: NM_001025366.2
• UniProt Accession #: P15692; O60720; O75875; Q074Z4; Q16889; B5BU86; H0Y2S8; H0Y407; H0Y414; H0Y462; H0Y8N2; H3BLW7
• Molecular Weight: 34,406 Da
• NCBI Official Full Name: vascular endothelial growth factor A isoform a
• NCBI Official Synonym Full Names: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VPF; VEGF; MVCD1
• NCBI Protein Information: vascular endothelial growth factor A; vascular permeability factor
• UniProt Protein Name: Vascular endothelial growth factor A
• Protein Family: VEGF coregulated chemokine
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF
• UniProt Entry Name: VEGFA_HUMAN

NCBI Description

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.