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Western Blot (WB) (Anti-FUT10 rabbit polyclonal antibody at 1:500 dilution Lane A: NCI-H292 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:56 kDa)

Rabbit anti-Human FUT10 Polyclonal Antibody | anti-FUT10 antibody

Anti-FUT10 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
FUT10; Polyclonal Antibody; Anti-FUT10 Antibody; Rabbit Polyclonal; FUT10 Antibody; Rabbit PAb; Antigen Affinity Purified; fucosyltransferase 10 (alpha (1; 3) fucosyltransferase); anti-FUT10 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FUT10
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-FUT10 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IP: 0.1-0.5uL/mg of lysate
Immunogen
Recombinant Human FUT10 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human FUT10 (rh FUT10). FUT10 specific IgG was purified by Human FUT10 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-FUT10 rabbit polyclonal antibody at 1:500 dilution Lane A: NCI-H292 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:56 kDa)

Western Blot (WB) (Anti-FUT10 rabbit polyclonal antibody at 1:500 dilution Lane A: NCI-H292 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:56 kDa Observed band size:56 kDa)

Immunoprecipitation (IP)

(FUT10 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 0.5 uL anti-FUT10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FUT10 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:5000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size :55 kDa)

Immunoprecipitation (IP) (FUT10 was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 0.5 uL anti-FUT10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-FUT10 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:5000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 56 kDa Observed band size :55 kDa)
References
Breton, C. et al., 1998, Glycobiology. 8 (1): 87-94. Oriol, R. et al., 1999, Glycobiology. 9 (4): 323-34. de Vries, T. et al., 2001, Glycobiology. 11 (10): 119R-128R. Baboval, T. et al., 2002, Mamm. Genome. 13: 538-541.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
56,094 Da
NCBI Official Full Name
FUT10 protein
NCBI Official Synonym Full Names
fucosyltransferase 10 (alpha (1,3) fucosyltransferase)
NCBI Official Symbol
FUT10
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 10; alpha-(1,3)-fucosyltransferase 10; fuc-TX; fucT-X; fucosyltransferase X; alpha 1,3-fucosyl transferase; galactoside 3-L-fucosyltransferase 10
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 10
UniProt Gene Name
FUT10
UniProt Synonym Gene Names
Fuc-TX; FucT-X
UniProt Entry Name
FUT10_HUMAN

Uniprot Description

FUT10: Probable fucosyltransferase. Belongs to the glycosyltransferase 10 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: Golgi apparatus; integral to membrane

Molecular Function: alpha(1,3)-fucosyltransferase activity

Biological Process: nervous system development; embryonic development; fertilization; wound healing; protein folding; protein amino acid glycosylation; cerebral cortex radially oriented cell migration; hemopoiesis; L-fucose catabolic process; protein targeting

Research Articles on FUT10

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Product Notes

The FUT10 fut10 (Catalog #AAA8105279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FUT10 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FUT10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.1-0.5uL/mg of lysate. Researchers should empirically determine the suitability of the FUT10 fut10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUT10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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