Chicken Interleukin 12, IL-12/70 ELISA Kit | IL-12/70 elisa kit

Chicken Interleukin 12, IL-12/70 ELISA Kit

Cat. Num. AAA700437

• Gene Names: IL12A; P35; CLMF; NFSK; NKSF1; IL-12A
• Reactivity: Chicken
• Synonyms: Interleukin 12; IL-12/70; Chicken Interleukin 12; IL-12/70 ELISA Kit; IL-12/70 elisa kit

• Reactivity: Chicken
• Sequence Length: 253

• Samples: Serum, plasma and tissue homogenates
• Detection Range: 0.78 pg/ml-200 pg/ml
• Sensitivity: 0.78 pg/ml
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-12/70 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-12/70. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-12/70 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-12/70, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-12/70 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4323579
• NCBI GeneID: 3592
• UniProt Accession #: P29459; Q96QZ1
• Molecular Weight: 24,874 Da
• NCBI Official Full Name: interleukin 12
• NCBI Official Synonym Full Names: interleukin 12A
• NCBI Official Symbol: IL12A
• NCBI Official Synonym Symbols: P35; CLMF; NFSK; NKSF1; IL-12A
• NCBI Protein Information: interleukin-12 subunit alpha; CLMF p35; IL35 subunit; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
• UniProt Protein Name: Interleukin-12 subunit alpha
• UniProt Gene Name: IL12A
• UniProt Synonym Gene Names: NKSF1; IL-12A; CLMF p35; NKSF1
• UniProt Entry Name: IL12A_HUMAN

NCBI Description

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.