Testing Data
(Published customer image Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.Image captionExpression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 ug/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities +/- SEM of 3 to 9 experiments for each condition.From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011) Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets. PLoS ONE 6(4): e18812.)
Mouse C3a RECEPTOR Monoclonal Antibody | anti-C3a antibody
MOUSE ANTI HUMAN C3a RECEPTOR
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20
Preparation
Fusion Partners
Shelf Life: 18 months from date of despatch.
Testing Data
(Published customer image Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.Image captionExpression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 ug/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities +/- SEM of 3 to 9 experiments for each condition.From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011) Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets. PLoS ONE 6(4): e18812.)
Testing Data
(Published customer image Mouse anti Human C3a receptor antibody, clone hC3aRZ1 used for flow cytometry.Image captionExpression of C3aR1 and C5aR on collagen- and convulxin-activated platelets. Washed platelets were activated for 10 min with 2.5 ug/mL collagen, 500 ng/mL convulxin or saline and further incubated for 30 min at RT and analyzed for C3aR1 and C5aR expression by flow cytometry. A: representative overlays of fluorescence histograms obtained for each condition using an alexa fluor 488 anti-C3aR1 (solid line), a FITC anti-CD88 (dashed line) or a FITC control IgG (dotted line). B: Mean fluorescence intensities +/- SEM of 3 to 9 experiments for each condition.From: Martel C, Cointe S, Maurice P, Matar S, Ghitescu M, et al. (2011) Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets. PLoS ONE 6(4): e18812.)
NCBI and Uniprot Product Information
Uniprot Description
C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; C3a anaphylatoxin receptor activity; complement component C3a receptor activity; phosphoinositide phospholipase C activity
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of angiogenesis; elevation of cytosolic calcium ion concentration; metabolic process; blood circulation; complement receptor mediated signaling pathway; inflammatory response; chemotaxis
Research Articles on C3a
Similar Products
Product Notes
The C3a c3ar1 (Catalog #AAA212809) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's C3a RECEPTOR can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the sugested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/20. Researchers should empirically determine the suitability of the C3a c3ar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3a RECEPTOR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
