Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Aldo-keto reductase family 1 member B10 (AKR1B10) Standard Curve.)
Human Aldo-keto Reductase Family 1 Member B10 (AKR1B10) ELISA Kit | AKR1B10 elisa kit
Human Aldo-keto Reductase Family 1 Member B10 (AKR1B10) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Aldo-keto reductase family 1 member B10 (AKR1B10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Aldo-keto reductase family 1 member B10 (AKR1B10) Standard Curve.)
Background: Aldose reductase (AR) is an NADPH-dependent enzyme that was first identified by its ability to reduce glucose to sorbitol. The deduced 316-amino acid AKR1B10 protein shares 71% amino acid identity with AR. Northern blot analysis revealed that AKR1B10 is overexpressed in 54% of HCCs, while AR is overexpressed in 29% of HCCs. Northern blot analysis of normal tissues showed that unlike the ubiquitously expressed AR, AKR1B10 is expressed most abundantly in small intestine and colon, with lower levels in liver, thymus, prostate, testis, and skeletal muscle. AR and ARL1 reduce a similar spectrum of aromatic and aliphatic aldehyde substrates.Highest expression of AKR1B10 in adrenal gland, with modest expression in stomach, placenta, small intestine, and pancreas, and lower expression in all other tissues tested.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
AKR1B10: Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. Belongs to the aldo/keto reductase family.
Protein type: EC 1.1.1.-; Lipid Metabolism - linoleic acid; EC 1.1.1.21; Carbohydrate Metabolism - butanoate; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose
Chromosomal Location of Human Ortholog: 7q33
Cellular Component: lysosome; cytosol
Molecular Function: protein binding; aldo-keto reductase activity; indanol dehydrogenase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity
Biological Process: steroid metabolic process; phototransduction, visible light; digestion; farnesol catabolic process; aldehyde metabolic process; retinoid metabolic process
Research Articles on AKR1B10
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Product Notes
The Human AKR1B10 akr1b10 (Catalog #AAA9714304) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714304 ELISA Kit recognizes Human AKR1B10. It is sometimes possible for the material contained within the vial of "Aldo-keto Reductase Family 1 Member B10 (AKR1B10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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