Mouse FELINE IMMUNODEFICIENCY VIRUS p24 gag Monoclonal Antibody
MOUSE ANTI FELINE IMMUNODEFICIENCY VIRUS p24 gag
Applications
Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Synonyms
FELINE IMMUNODEFICIENCY VIRUS p24 gag; Monoclonal Antibody; MOUSE ANTI FELINE IMMUNODEFICIENCY VIRUS p24 gag; anti-FELINE IMMUNODEFICIENCY VIRUS p24 gag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
PAK3-2C1
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-FELINE IMMUNODEFICIENCY VIRUS p24 gag antibody
Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Western Blot (WB)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Perservative Stabilisers
0.05% Sodium Azide
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Viral
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-FELINE IMMUNODEFICIENCY VIRUS p24 gag antibody
Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 recognizes an immunodominant p24 epitope of feline immunodeficiency virus (FIV) p24 gag. The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. The specific binding epitope recognized by Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 appears to be enclosed in a tight peptide coil during gag production and maturation. The addition of 0.3% Triton-X detergent is recommended to reveal this epitope for antibody binding. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 detects a dominant band of approximately 24kDa, and also detects p24 gag precursor bands at 36, 39, 49 and 52kDa under reducing conditions in Western blotting. Mouse anti Feline Immunodeficiency Virus p24 gag antibody, clone PAK3-2C1 does not recognize Feline Leukaemia Virus (FeLV), Feline Herpes Virus (FHV) type 1, Feline Coronavirus or Feline Calicivirus.
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Product Notes
The FELINE IMMUNODEFICIENCY VIRUS p24 gag (Catalog #AAA212642) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FELINE IMMUNODEFICIENCY VIRUS p24 gag can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Western Blot (WB). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. Flow Cytometry: Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the FELINE IMMUNODEFICIENCY VIRUS p24 gag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FELINE IMMUNODEFICIENCY VIRUS p24 gag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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