Testing Data
(Staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MBS214991))
Mouse FIBROBLASTS/EPITHELIAL CELLS Monoclonal Antibody
MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
FIBROBLASTS/EPITHELIAL CELLS; Monoclonal Antibody; MOUSE ANTI HUMAN FIBROBLASTS/EPITHELIAL CELLS; anti-FIBROBLASTS/EPITHELIAL CELLS antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
D7-FIB
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Applicable Applications for anti-FIBROBLASTS/EPITHELIAL CELLS antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Immunohistology - Frozen: Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Immunogen
Human foreskin fibroblasts
Fusion Partners
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MBS214991))
Testing Data
(Staining of KG1 cells with Mouse anti Human Fibroblasts/ Epithelial cells (MBS214991))
Related Product Information for anti-FIBROBLASTS/EPITHELIAL CELLS antibody
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies Mouse anti Human Fibroblasts/Epithelial Cells antibody. Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001). Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB is routinely tested in flow cytometry on the KG1 cell line.
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Product Notes
The FIBROBLASTS/EPITHELIAL CELLS (Catalog #AAA214992) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's FIBROBLASTS/EPITHELIAL CELLS can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Maximum Dilution: 1/100; Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the FIBROBLASTS/EPITHELIAL CELLS for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FIBROBLASTS/EPITHELIAL CELLS, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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