Rabbit anti-Mouse Aconitase 1 (ACO1) Polyclonal Antibody | anti-ACO1 antibody

Cy3-Linked Polyclonal Antibody to Aconitase 1 (ACO1)

Cat. Num. AAA2110698

• Gene Names: Aco1; Irp1; Aco-1; Irebp
• Reactivity: Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Aconitase 1 (ACO1); Polyclonal Antibody; Cy3-Linked Polyclonal Antibody to Aconitase 1 (ACO1); IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein; anti-ACO1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a Rabbit polyclonal antibody raised against ACO1. It has been selected for its ability to recognize ACO1 in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid
• Concentration: >=100ug/ml (varies by lot)
• Sequence: RPJ683Mu03-Recombinant Aconitase 1 (ACO1)
• Sequence Length: 889

• Applicable Applications for anti-ACO1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Conjugation: Cy3
• Cross Reactivity: Mouse
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; ; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Knockout Validation

(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: ACO1 knockout HepG2 cell lysate; Predicted MW: 98kd Observed MW: 100kd Primary Ab: 1ug/ml Rabbit Anti-Mouse ACO1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

NCBI and Uniprot Product Information

• NCBI GI #: 110347487
• NCBI GeneID: 11428
• NCBI Accession #: NP_031412.2
• NCBI GenBank Nucleotide #: NM_007386.2
• UniProt Accession #: P28271
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1
• NCBI Official Symbol: Aco1
• NCBI Official Synonym Symbols: Irp1; Aco-1; Irebp
• NCBI Protein Information: cytoplasmic aconitate hydratase
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: Aco1
• UniProt Synonym Gene Names: Ireb1; Irebp; Aconitase; IRP1; IRE-BP 1
• UniProt Entry Name: ACOC_MOUSE

NCBI Description

This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014]