Aconitase 1 (ACO1) Recombinant Protein | ACO1 recombinant protein

Recombinant Aconitase 1 (ACO1)

Cat. Num. AAA2012385

• Gene Names: Aco1; Irp1; Aco-1; Irebp
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aconitase 1 (ACO1); Recombinant Aconitase 1 (ACO1); ACO1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-VAVG VLSGNRNFEG RVHPNTRANY LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GRQVFLKDIW PTRDEIQAVE RQHVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE SLTLDLQPPK SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG LTPREFNSYG SRRGNDAIM
• Sequence Length: 889

• Applicable Applications for ACO1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Val527~Met719 (Accession # P28271) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 110347487
• NCBI GeneID: 11428
• NCBI Accession #: NP_031412.2
• NCBI GenBank Nucleotide #: NM_007386.2
• UniProt Accession #: P28271; Q3TQ15; Q99K54
• Molecular Weight: 23.0kDa
• NCBI Official Full Name: cytoplasmic aconitate hydratase
• NCBI Official Synonym Full Names: aconitase 1
• NCBI Official Symbol: Aco1
• NCBI Official Synonym Symbols: Irp1; Aco-1; Irebp
• NCBI Protein Information: cytoplasmic aconitate hydratase; IRE-BP 1; citrate hydro-lyase; cytoplasmic aconitase; iron regulatory protein 1; iron-responsive element-binding protein 1
• UniProt Protein Name: Cytoplasmic aconitate hydratase
• Protein Family: Aconitate hydratase
• UniProt Gene Name: Aco1
• UniProt Synonym Gene Names: Ireb1; Irebp; Aconitase; IRP1; IRE-BP 1
• UniProt Entry Name: ACOC_MOUSE

NCBI Description

This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014]

Uniprot Description

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.

Protein type: EC 4.2.1.3; Endoplasmic reticulum; Carbohydrate Metabolism - citrate (TCA) cycle; Lyase; Carbohydrate Metabolism - glyoxylate and dicarboxylate; RNA-binding; Translation

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; cell; endoplasmic reticulum; cytoplasm; cytosol

Molecular Function: iron-responsive element binding; RNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; lyase activity; iron-sulfur cluster binding; aconitate hydratase activity; mRNA 5'-UTR binding

Biological Process: regulation of translation; regulation of gene expression; cellular iron ion homeostasis; metabolic process; tricarboxylic acid cycle; citrate metabolic process; response to iron(II) ion; intestinal absorption; post-embryonic development