Western Blot (WB)
(Western Blot:Sample: Recombinant ACO1, Mouse.)
Aconitase 1 Polyclonal Antibody | anti-ACO1 antibody
Polyclonal Antibody to Aconitase 1 (ACO1)
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Keep in Dark Place
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Recombinant ACO1, Mouse.)
Western Blot (WB)
(Western Blot:Sample: Recombinant ACO1, Mouse.)
Western Blot (WB)
(Western Blot;Sample: Mouse Liver lysate;Primary Ab: 1.5ug/ml Rabbit Anti-Mouse ACO1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Western Blot (WB)
(Western Blot;Sample: Mouse Liver lysate;Primary Ab: 1.5ug/ml Rabbit Anti-Mouse ACO1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Immunohistochemistry (IHC)
(DAB staining on IHC-PSamples Mouse Stomach Tissue;Primary Ab: 30ug/mL Anti-Mouse AC01 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
Immunohistochemistry (IHC)
(DAB staining on IHC-PSamples Mouse Stomach Tissue;Primary Ab: 30ug/mL Anti-Mouse AC01 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047) )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism. Disruption of this gene is associated with pulmonary hypertension and polycythemia. [provided by RefSeq, Jan 2014]
Uniprot Description
IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family.
Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 4.2.1.3; Endoplasmic reticulum; Lyase; RNA-binding; Translation
Chromosomal Location of Human Ortholog: 4 A5|4 20.24 cM
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; extracellular exosome; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion
Molecular Function: 3 iron, 4 sulfur cluster binding; 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; iron-sulfur cluster binding; lyase activity; metal ion binding; mRNA 5'-UTR binding; RNA binding
Biological Process: cellular iron ion homeostasis; citrate metabolic process; intestinal absorption; metabolic process; post-embryonic development; regulation of gene expression; regulation of translation; response to iron(II) ion; tricarboxylic acid cycle
Research Articles on ACO1
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Product Notes
The ACO1 aco1 (Catalog #AAA2028596) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Aconitase 1 (ACO1) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Aconitase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ACO1 aco1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aconitase 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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