Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit | TDP1 elisa kit

Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit

Cat. Num. AAA2098010

• Reactivity: Human
• Synonyms: Tyrosyl DNA Phosphodiesterase 1 (TDP1); Human Tyrosyl DNA Phosphodiesterase 1 (TDP1) ELISA Kit; SCAN1; Tyr-DNA phosphodiesterase 1; TDP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tyrosyl DNA Phosphodiesterase 1 (TDP1).; No significant cross-reactivity or interference between Tyrosyl DNA Phosphodiesterase 1 (TDP1) and analogues was observed.
• Sequence Length: 609

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosyl DNA Phosphodiesterase 1 (TDP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosyl DNA Phosphodiesterase 1 (TDP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TDP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosyl DNA Phosphodiesterase 1 (TDP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosyl DNA Phosphodiesterase 1 (TDP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosyl DNA Phosphodiesterase 1 (TDP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosyl DNA Phosphodiesterase 1 (TDP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TDP1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 72255547
• NCBI GeneID: 314380
• NCBI Accession #: NP_001026827.1
• NCBI GenBank Nucleotide #: NM_001031657.1
• UniProt Accession #: Q4G056
• Molecular Weight: 68,632 Da
• NCBI Official Full Name: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Symbol: Tdp1
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 1
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 1
• Protein Family: Tyrosyl-DNA phosphodiesterase
• UniProt Gene Name: Tdp1
• UniProt Synonym Gene Names: Tyr-DNA phosphodiesterase 1

Uniprot Description

TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.

Protein type: DNA repair, damage; EC 3.1.4.-; Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q32

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: double-stranded DNA binding; exonuclease activity; single-stranded DNA binding; tyrosyl-DNA phosphodiesterase activity

Biological Process: DNA repair; double-strand break repair; single strand break repair