Rat Matrix Extracellular Phosphoglycoprotein (MEPE) DIY ELISA Kit | MEPE diy elisa kit
Rat Matrix Extracellular Phosphoglycoprotein (MEPE) ELISA Kit DIY Materials
Gene Names
MEPE; OF45
Reactivity
Rat
Synonyms
Matrix Extracellular Phosphoglycoprotein (MEPE); Rat Matrix Extracellular Phosphoglycoprotein (MEPE) ELISA Kit DIY Materials; OF45; Asarm Motif-Containing; Osteoblast/Osteocyte Factor 45; MEPE diy elisa kit
Reactivity
Rat
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Matrix Extracellular Phosphoglycoprotein (MEPE). No significant cross-reactivity or interference between Matrix Extracellular Phosphoglycoprotein (MEPE) and analogues was observed.
Sequence Length
525
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma and other biological fluids.
Detection Range
1.56-100ng/mL
Sensitivity
0.62ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for MEPE diy elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,291 Da
NCBI Official Full Name
matrix extracellular phosphoglycoprotein isoform a
NCBI Official Synonym Full Names
matrix extracellular phosphoglycoprotein
NCBI Official Symbol
MEPE
NCBI Official Synonym Symbols
OF45
NCBI Protein Information
matrix extracellular phosphoglycoprotein
UniProt Protein Name
Matrix extracellular phosphoglycoprotein
Protein Family
UniProt Gene Name
MEPE
UniProt Synonym Gene Names
OF45
NCBI Description
This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Uniprot Description
MEPE: Promotes renal phosphate excretion and modulates mineralization. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q22.1
Molecular Function: extracellular matrix structural constituent; protein binding
Biological Process: biomineral formation; skeletal development
Research Articles on MEPE
1. Results indicate an association of MEPE gene inactivation with decreased survival after DNA damage.
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Product Notes
The Rat MEPE mepe (Catalog #AAA2089054) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089054 DIY ELISA Kit recognizes Rat MEPE. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the MEPE mepe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matrix Extracellular Phosphoglycoprotein (MEPE), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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